The skin of atopic dermatitis patients contains a novel enzyme, glucosylceramide sphingomyelin deacylase, which cleaves the N-acyl linkage of sphingomyelin and glucosylceramide.
The skin of atopic dermatitis patients contains a novel enzyme, glucosylceramide sphingomyelin deacylase, which cleaves the N-acyl linkage of sphingomyelin and glucosylceramide.
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特应性皮炎患者的皮肤含有一种新型酶——葡萄糖神经酰胺鞘磷脂脱酰酶，它可以裂解鞘磷脂和葡萄糖神经酰胺的N-酰基键。
DOI：
10.1042/bj3500747
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
G. Imokawa
中科院分区：
文献类型：
--
作者：
Kazuhiko Higuchi;Junko Hara;Reiko Okamoto;Makoto Kawashima;G. Imokawa
我们以前已经证明，有一个异常的表达鞘磷脂（SM）脱酰酶在特应性皮炎（ADe）患者的表皮。在本研究中，我们制备了N-[棕榈酸-1-（14）C]SM和N-[棕榈酸-1-（14）C]葡萄糖神经酰胺（GCer）用作底物，并通过检测AD患者角质层或表皮提取物中[（14）C]棕榈酸的释放来定量SM脱酰酶活性。在以[棕榈酸-1-（14）C]SM为底物的研究中，发现SM脱酰酶的催化活性与pH值有关，在pH 5.0处有一个峰值，用ADe表皮提取物进行的SDS/PAGE分析表明SM脱酰酶的分子量为40000 Da，这与角质层提取物凝胶过滤分析估计的42000 Da的表观分子量一致。等电聚焦（IEF）分析表明，SM脱酰酶、β-葡萄糖脑苷脂酶（GlcCDase）、鞘磷脂酶（SMase）和酸性神经酰胺酶的pI值分别为4.2、7.4、7.0和5.7。在使用通过IEF部分纯化的pI-4.2 SM脱酰基酶（未检出酸性神经酰胺酶、GlcCD酶或SM酶污染）进行的酶分析中，放射性TLC分析显示，放射性标记的鞘氨醇磷酸胆碱或[1-（14）C]棕榈酸分别从用作底物的[胆碱-甲基-（14）C]SM或N-[棕榈酰-1-（14）C]GCer中酶促释放。此外，从AD患者角质层提取物中纯化的pI-4.2蛋白能够水解N-[棕榈酰-1-（14）C]SM和GCer，但不能水解N-[棕榈酰-1-（14）C]神经酰胺。这些结果表明，迄今未发现的表皮酶，在这里被称为葡糖神经酰胺鞘磷脂脱酰酶，是在皮肤中表达的AD e患者，其中起着重要的作用，在角质层中的神经酰胺缺乏症（包括酰基神经酰胺）。
We have demonstrated previously that there is an abnormal expression of sphingomyelin (SM) deacylase in the epidermis of patients with atopic dermatitis (ADe). In the present study, we have prepared N-[palmitic acid-1-(14)C]SM and N-[palmitic acid-1-(14)C]glucosylceramide (GCer) to use as substrates and have quantified SM deacylase activity by detecting the release of [(14)C]palmitic acid in extracts of the stratum corneum or the epidermis of ADe patients. In studies using [palmitic acid-1-(14)C]SM as a substrate, a pH dependency of catalytic activity with a peak at pH 5.0 was found. Preparative SDS/PAGE using an extract of ADe epidermis revealed that the molecular mass of SM deacylase is 40000 Da, which is consistent with its apparent molecular mass of 42000 Da estimated by gel-filtration analysis of stratum corneum extracts. Analytical isoelectric focusing (IEF) chromatography demonstrated that the pI values of SM deacylase, beta-glucocerebrosidase (GlcCDase), sphingomyelinase (SMase) and acid ceramidase were 4.2, 7.4, 7.0 and 5.7, respectively. In enzymic analysis using pI-4.2 SM deacylase partially purified by IEF, which had no detectable contamination with acid ceramidase, GlcCDase or SMase, radio-TLC analysis revealed that radiolabelled sphingosylphosphocholine or [1-(14)C]palmitic acid was enzymically liberated from [choline-methyl-(14)C]SM or N-[palmitoyl-1-(14)C]GCer, respectively, used as substrates. Further the pI-4.2 protein purified from extracts of the stratum corneum of ADe patients was able to hydrolyse N-[palmitoyl-1-(14)C]SM and GCer, but not N-[palmitoyl-1-(14)C]ceramide. These results indicate that a hitherto undiscovered epidermal enzyme, termed here glucosylceramide sphingomyelin deacylase, is expressed in the skin of ADe patients, which plays an important role in ceramide deficiency (including acylceramides) in the stratum corneum.
四川省石鹧鸪菌三新种（鞘翅目、葡萄科、 Paederinae）
影响因子：
1.3
作者：
Peng Z;Li LZ;Zhao MJ
通讯作者：
Zhao MJ
人白血病 (HL-60) 细胞神经酰胺激酶活性的表征。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kolesnick,RN;Hemer,MR
通讯作者：
Hemer,MR
脂质含量和脂质类型作为表皮渗透屏障的决定因素。
影响因子：
6.5
作者：
Grubauer,G;Feingold,KR;Harris,RM;Elias,PM
通讯作者：
Elias,PM