A biomimetic hydrogel based on methacrylated dextran-graft-lysine and gelatin for 3D smooth muscle cell culture
A biomimetic hydrogel based on methacrylated dextran-graft-lysine and gelatin for 3D smooth muscle cell culture
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DOI：
10.1016/j.biomaterials.2009.10.040
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发表时间：
2010-02-01
期刊：
影响因子：
14
通讯作者：
Chan-Park, Mary B.
中科院分区：
文献类型：
--
作者：
Liu, Yunxiao;Chan-Park, Mary B.
迄今为止，许多用于细胞封装的合成水凝胶都是基于聚乙二醇，聚乙二醇是非天然的、不可生物降解的并且只能末端官能化，所有这些都是组织工程或细胞递送的缺点。多糖葡聚糖也是高度亲水性的，但可生物降解和悬垂官能化，并且更类似于糖胺聚糖以模拟天然细胞外基质。本研究报告的甲基丙烯酸酯和赖氨酸功能化的葡聚糖的合成和水凝胶复合系统的开发基于这种材料和甲基丙烯酰胺改性明胶。通过操纵葡聚糖和明胶的官能化程度以及前体溶液的浓度/组成来改变水凝胶的机械刚度和溶胀度。人脐动脉平滑肌细胞（SMCs）被封装在水凝胶内，在凝胶硬化与光聚合。快速细胞铺展，广泛的细胞网络形成和高SMC增殖发生在较软的水凝胶（剪切储能模量范围从898至3124 Pa）。由于水凝胶施加的物理约束，包封的SMC在初始培养物中比在组织培养聚苯乙烯皿上似乎相对收缩，但它们随着时间的推移变得更合成，这可能是由于细胞不能在这些细胞介导的可降解水凝胶内达到汇合。从令人印象深刻的细胞增殖和网络形成，这些新的水凝胶结合多糖和蛋白质衍生物似乎是进一步开发的生物活性支架用于血管组织工程和再生的优秀候选人。(C)2009爱思唯尔有限公司保留所有权利。
Many synthetic hydrogels for cell encapsulation have hitherto been based on polyethylene glycol which is non-natural, non-biodegradable and only terminal-functionalizable, all of which are drawbacks for tissue engineering or cell delivery. The polysaccharide dextran is also highly hydrophilic but biodegradable and pendant-functionalizable and more closely resembles glycosaminoglycans to mimic the natural extracellular matrix. This study reports synthesis of a methacrylate and lysine functionalized dextran and development of hydrogel composite systems based on this material and methacrylamide modified gelatin. The mechanical stiffness and degree of swelling of the hydrogels were varied by manipulation of the degree of functionalization of dextran and gelatin and concentration/composition of precursor solution. Human umbilical artery smooth muscle cells (SMCs) were encapsulated inside hydrogels during gel hardening with photopolymerization. Rapid cell spreading, extensive cellular network formation and high SMC proliferation occurred within softer hydrogels (with shear storage moduli ranging from 898 to 3124 Pa). The encapsulated SMCs appear to be relatively contractile in the initial culture than on tissue culture polystyrene dish due to physical constraint imposed by the hydrogels but they become more synthetic with time possibly due to the inability of cells to reach confluence inside these cell-mediated degradable hydrogels. From the impressive cell proliferation and network formation, these new hydrogels combining polysaccharide and protein derivatives appear to be excellent candidates for further development as bioactive scaffolds for use in vascular tissue engineering and regeneration. (C) 2009 Elsevier Ltd. All rights reserved.