Rescue of influenza A virus from recombinant DNA
Rescue of influenza A virus from recombinant DNA
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DOI：
10.1128/jvi.73.11.9679-9682.1999
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发表时间：
1999-11-01
影响因子：
5.4
通讯作者：
García-Sastre, A
中科院分区：
文献类型：
--
作者：
Fodor, E;Devenish, L;García-Sastre, A
我们通过将12个质粒转染到Vero细胞中拯救了甲型流感病毒。这8个单独的负义基因组病毒RNA是从含有人RNA聚合酶I启动子和丁型肝炎病毒核酶序列的质粒转录的。从蛋白质表达质粒表达三种流感病毒聚合酶蛋白和核蛋白。这种基于质粒的反向遗传学技术有助于产生基因中含有特定突变的重组流感病毒。
We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.