DNA gyrase binds to the family of prokaryotic repetitive extragenic palindromic sequences.
DNA gyrase binds to the family of prokaryotic repetitive extragenic palindromic sequences.
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DNA 旋转酶与原核重复基因外回文序列家族结合。
DOI：
10.1073/pnas.85.23.8850
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发表时间：
1988
影响因子：
11.1
通讯作者：
Ames,GF
中科院分区：
文献类型：
--
作者：
Yang,Y;Ames,GF
一个重复的基因外回文(REP)序列家族由分布在整个染色体上的数百个拷贝组成。它们的回文性质和保守性表明它们是由一种蛋白质专门识别的(S)。我们已经确定DNA旋转酶[DNA拓扑异构酶(ATP-水解酶，EC 5.99.1.3)]是Rep结合蛋白之一。与不含REP序列的DNA相比，旋转酶对含有REP序列的DNA的亲和力至少高10倍。结合效果与DNA中REP序列的数量直接相关。DNase I足迹显示，旋转酶保护包裹REP序列的含有REP的DNA片段上的205个碱基对。与上述结果一致，将REP共有序列与先前确定的pBR322“强”旋转酶切割位点序列进行比较，显示出高度的同源性。由于REP序列在基因组中大量存在，我们认为它们通过与旋转酶的相互作用而具有生理功能，例如，它们是维持DNA超螺旋的作用部位。此外，我们推测这些相互作用可能是结构性的，例如参与细菌染色体的高阶结构。
A family of repetitive extragenic palindromic (REP) sequences is composed of hundreds of copies distributed throughout the chromosome. Their palindromic nature and conservation suggested that they are specifically recognized by a protein(s). We have identified DNA gyrase [DNA topoisomerase (ATP-hydrolysing), EC 5.99.1.3] as one of the REP-binding proteins. Gyrase has at least a 10-fold higher affinity for DNA containing REP sequences than for DNA not containing REP sequences. Binding effectiveness correlates directly with the number of REP sequences in the DNA. DNase I footprinting shows that gyrase protects 205 base pairs on a REP-containing DNA fragment enclosing the REP sequences. In agreement with the above results, a comparison of the REP consensus sequence with the sequence of previously identified pBR322 "strong" gyrase cleavage sites reveals a high degree of homology. Because REP sequences are numerous and found throughout the genome, we suggest they have physiological functions mediated through their interaction with gyrase, such as being sites of action for the maintenance of DNA supercoiling. In addition, we speculate that these interactions may be of a structural nature, such as involvement in the higher-order structure of the bacterial chromosome.