The reduction of reactive oxygen species formation by mitochondrial alternative respiration in tomato basal defense against TMV infection
The reduction of reactive oxygen species formation by mitochondrial alternative respiration in tomato basal defense against TMV infection
复制标题
番茄抗TMV感染的基础防御中线粒体替代呼吸减少活性氧的形成
DOI：
10.1007/s00425-011-1483-z
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发表时间：
2012-02-01
期刊：
影响因子：
4.3
通讯作者：
Yu, Jing-Quan
中科院分区：
文献类型：
--
作者：
Liao, Yang-Wen-Ke;Shi, Kai;Yu, Jing-Quan
研究了番茄体内线粒体交替氧化酶(AOX)的作用及其与系统AOX诱导、ROS形成和植物对烟草花叶病毒(TMV)基础防御的关系。结果表明，接种烟草花叶病毒显著提高了未接种的上部叶片的AOX基因转录本水平、泛醌还原水平、丙酮酸含量和抗氰呼吸(抗CN呼吸)。氰化钾(KCN，细胞色素途径抑制剂)可显著增加植物对CN的抗性，减少ROS的形成，而AOX抑制剂水杨基异羟肟酸(SHAM)可抑制AOX活性，促进ROS的产生。此外，与未处理的TMV相比，SHAM增强了TMV的系统侵染，而KCN处理则降低了TMV的系统侵染。此外，KCN处理显著降低了TMV诱导的抗氧化酶活性和脱氢抗坏血酸/总抗坏血酸含量的增加，而假处理植株的变化则相反。这些结果表明，线粒体AOX途径的全身性诱导在降低ROS以增强基础防御中起着关键作用。在维持细胞氧化还原动态平衡的过程中，其他的抗氧化剂系统也被协调调节。
The role of mitochondrial alternative oxidase (AOX) and the relationship between systemic AOX induction, ROS formation, and systemic plant basal defense to Tobacco mosaic virus (TMV) were investigated in tomato plants. The results showed that TMV inoculation significantly increased the level ofAOXgene transcripts, ubiquinone reduction levels, pyruvate content, and cyanide-resistant respiration (CN-resistant R) in upper, un-inoculated leaves. Pretreatment with potassium cyanide (KCN, a cytochrome pathway inhibitor) greatly increased CN-resistant R and reduced reactive oxygen species (ROS) formation, while application of salicylhydroxamic acid (SHAM, an AOX inhibitor) blocked the AOX activity and enhanced the production of ROS in the plants. Furthermore, TMV systemic infection was enhanced by SHAM and reduced by KCN pretreatment, as compared with the un-pretreated TMV counterpart. In addition, KCN application significantly diminished TMV-induced increase in antioxidant enzyme activities and dehydroascorbate/total ascorbate pool, while an opposite change was observed with SHAM-pretreated plants. These results suggest that the systemic induction of the mitochondrial AOX pathway plays a critical role in the reduction of ROS to enhance basal defenses. Additional antioxidant systems were also coordinately regulated in the maintenance of the cellular redox homeostasis.