Fluorescence multispectral imaging-based diagnostic system for atherosclerosis.
Fluorescence multispectral imaging-based diagnostic system for atherosclerosis.
复制标题
DOI：
10.1186/s12938-016-0220-z
复制
发表时间：
2016-08-20
影响因子：
3.9
通讯作者：
Morimoto Y
中科院分区：
文献类型：
--
作者：
Ho CS;Horiuchi T;Taniguchi H;Umetsu A;Hagisawa K;Iwaya K;Nakai K;Azmi A;Zulaziz N;Azhim A;Shinomiya N;Morimoto Y
与正常动脉壁不同，动脉粥样硬化的动脉壁含有丰富的脂质，如胆固醇。在这项研究中，我们的目标是通过多光谱荧光成像来利用这种差异来诊断动脉粥样硬化，这使得能够识别源自动脉壁物质的荧光。提取的动脉(兔腹主动脉、人冠状动脉)内表面用405 nm激发光照射，获得多光谱荧光图像。对人冠状动脉标本进行病理检查，通过测量中膜厚度和内膜厚度计算冠状动脉厚度。动脉粥样硬化部位的荧光光谱与正常部位不同。在每个样本中选择多个感兴趣区域(ROI)，并确定来自每个ROI的两个荧光强度差(其中每个强度差是在识别器波长和基础波长之间计算的)之间的比率，从而允许区分动脉粥样硬化部位。荧光强度与动脉厚度呈显著正相关。这些结果表明，多光谱荧光成像提供了动脉粥样硬化的定性和定量评估，因此是诊断疾病的一种可行的方法。本文的在线版本(doi：10.1186/s12938-0160220-z)包含补充材料，授权用户可以使用。
Composition of atherosclerotic arterial walls is rich in lipids such as cholesterol, unlike normal arterial walls. In this study, we aimed to utilize this difference to diagnose atherosclerosis via multispectral fluorescence imaging, which allows for identification of fluorescence originating from the substance in the arterial wall. The inner surface of extracted arteries (rabbit abdominal aorta, human coronary artery) was illuminated by 405 nm excitation light and multispectral fluorescence images were obtained. Pathological examination of human coronary artery samples were carried out and thickness of arteries were calculated by measuring combined media and intima thickness. The fluorescence spectra in atherosclerotic sites were different from those in normal sites. Multiple regions of interest (ROI) were selected within each sample and a ratio between two fluorescence intensity differences (where each intensity difference is calculated between an identifier wavelength and a base wavelength) from each ROI was determined, allowing for discrimination of atherosclerotic sites. Fluorescence intensity and thickness of artery were found to be significantly correlated. These results indicate that multispectral fluorescence imaging provides qualitative and quantitative evaluations of atherosclerosis and is therefore a viable method of diagnosing the disease. The online version of this article (doi:10.1186/s12938-016-0220-z) contains supplementary material, which is available to authorized users.