In Vivo Energy Transfer from Bacteriochlorophyll c, d, e, or f to Bacteriochlorophyll a in Wild‐Type and Mutant Cells of the Green Sulfur Bacterium Chlorobaculum limnaeum
In Vivo Energy Transfer from Bacteriochlorophyll c, d, e, or f to Bacteriochlorophyll a in Wild‐Type and Mutant Cells of the Green Sulfur Bacterium Chlorobaculum limnaeum
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DOI：
10.1002/cptc.201700164
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发表时间：
2018-03
期刊：
影响因子：
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通讯作者：
J. Harada;Y. Shibata;Misato Teramura;T. Mizoguchi;Yusuke Kinoshita;Ken Yamamoto;H. Tamiaki
中科院分区：
文献类型：
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作者：
J. Harada;Y. Shibata;Misato Teramura;T. Mizoguchi;Yusuke Kinoshita;Ken Yamamoto;H. Tamiaki
绿硫细菌具有光收集天线系统，称为叶绿体，根据菌株的不同，叶绿体通常含有细菌叶绿素（BChl） c、d和e分子中的一种。此外，BChl - f从未在自然界中发现，但在构建的突变体的叶绿体中观察到。在本研究中，我们利用含有BChl e的棕绿色硫细菌Chlorobaculum limnaeum RK-j-1菌株，构建了只积累BChl c、d和f的突变体。在突变体细胞中，这些色素表现出不同的吸收光谱，其Qy峰按BChl c、d、e、f.通过皮秒时间分辨荧光测量，在77 K下观察到这些突变细胞中从叶绿体聚集体到bcl -a的能量转移。根据Forster能量传递机制，从叶绿体聚集体到BChl a的能量传递效率依次为BChl c、d、e和f。
Green sulfur bacteria have light-harvesting antenna systems, called chlorosomes, which usually contain one of bacteriochlorophyll (BChl) c, d, and e molecules depending on the bacterial strain. Additionally, BChl f has been never found in nature but observed in chlorosomes of the constructed mutant. In this study, we used the brown-colored green sulfur bacterium Chlorobaculum limnaeum RK-j-1 strain possessing BChl e, and constructed its mutants accumulating only either BChl c, d, or f. In the mutant cells, these pigments showed different absorption spectra, and their Qy peaks shifted hypsochromically in the order of BChls c, d, e, and f. The energy-transfer from the chlorosomal aggregates to BChl-a in these mutant cells was observed at 77 K by using the picosecond time-resolved fluorescence measurements. According to the Forster energy transfer mechanism, the energy-transfer efficiency from the chlorosomal aggregates to BChl a was higher in the order of BChls c, d, e, and f.