Mouse models of liver cancer: Progress and recommendations.
Mouse models of liver cancer: Progress and recommendations.
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肝癌小鼠模型：进展与建议
DOI：
10.18632/oncotarget.4202
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发表时间：
2015-09-15
期刊：
影响因子：
--
通讯作者：
He XX
中科院分区：
文献类型：
--
作者：
He L;Tian DA;Li PY;He XX
为了阐明肝细胞癌（HCC）的发病机制和研究潜在的治疗效果，已经开发了许多小鼠模型。皮下异种移植模型在过去的几十年中被广泛使用。然而，随着体内成像技术的出现，如今研究人员越来越关注原位模型。基因工程小鼠模型（GEM）极大地促进了肝癌发生中基因功能的研究。最近，miR-122和miR-221的GEM为更好地理解microRNA在肝癌发生中的体内功能提供了新的途径。化学诱导的动物肝肿瘤具有许多人类HCC的形态学、组织发生学和生物化学特征。然而，复杂和模糊的基因组变异限制了它们的应用。本文对目前常用的小鼠模型和一些新出现的模型进行了综述，并着重分析了它们的优缺点，为肝癌研究者选择“最合适”的动物模型提供了参考。
To clarify the pathogenesis of hepatocellular carcinoma (HCC) and investigate the effects of potential therapies, a number of mouse models have been developed. Subcutaneous xenograft models are widely used in the past decades. Yet, with the advent of in vivo imaging technology, investigators are more and more concerned with the orthotopic models nowadays. Genetically engineered mouse models (GEM) have greatly facilitated studies of gene function in HCC development. Recently, GEM of miR-122 and miR-221 provided new approaches for better understanding of the in vivo functions of microRNA in hepatocarcinogenesis. Chemically induced liver tumors in animals share many of the morphological, histogenic, and biochemical features of human HCC. Yet, the complicated and obscure genomic alternation restricts their applications. In this review, we highlight both the frequently used mouse models and some emerging ones with emphasis on their merits or defects, and give advises for investigators to chose a “best-fit” animal model in HCC research.