Bile acid stimulation of cyclic AMP and ion transport in developing rabbit colon.
Bile acid stimulation of cyclic AMP and ion transport in developing rabbit colon.
复制标题
胆汁酸刺激发育中的兔结肠中的环 AMP 和离子转运。
DOI：
10.1097/00005176-199111000-00001
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发表时间：
1991
影响因子：
2.9
通讯作者：
Burlingame,SM
中科院分区：
文献类型：
--
作者：
Potter,GD;Sellin,JH;Burlingame,SM
胆汁酸可引起成年兔结肠远端氯的分泌和短路电流(ISC)的增加，而新生动物则不能。在本研究中，我们发现低至50μM的牛磺酸脱氧胆酸可显着增加成人结肠的环磷酸腺苷(CAMP)含量，但对新生大鼠的cAMP无明显影响。此外，用3，4，5-三甲氧基苯甲酸酯8-(N，N-二甲氨基)辛酯(TMB-8)阻断成人结肠cAMP的增加，可部分抑制TDC对氯离子分泌的影响。TMB-8不能阻断血管活性肠肽(VIP)、茶碱或Forsklin引起的cAMP升高。当新生儿结肠暴露于1 mM的TDC时，氯的分泌受到限制。在缺乏TDC诱导分泌的新生儿结肠中，cAMP未见增加。因此，cAMP的增加可能是TDC刺激与氯分泌偶联的一部分。
Bile acids elicit Cl secretion and increases in short circuit current (Isc) in rabbit distal colon in vitro in adult but not newborn animals. In this investigation we found that concentrations of taurodeoxycholic acid (TDC) as low as 50 μM significantly increase cyclic AMP (cAMP) in adult but not newborn colon. Further, blocking the increases of cAMP in adult colon with 3,4,5‐trimethoxybenzoate 8‐(N,N‐dimethylamino) octyl ester (TMB‐8), partially inhibited the effect of TDC on Cl secretion. TMB‐8 did not block the effect of increases in cAMP seen with vasoactive intestinal peptide (VIP), the‐ophylline, or forskolin. When newborn colon was exposed to 1 mM TDC, limited Cl secretion was elicited. Increased cAMP is not seen in newborn colon where TDC‐induced secretion is absent. Thus, increases in cAMP may represent one part of the coupling of TDC stimulation to Cl secretion.