SEQUENCES OF MESSENGER-RNAS DERIVED FROM GENOME RNA SEGMENT 7 OF INFLUENZA-VIRUS - COLINEAR AND INTERRUPTED MESSENGER-RNAS CODE FOR OVERLAPPING PROTEINS
SEQUENCES OF MESSENGER-RNAS DERIVED FROM GENOME RNA SEGMENT 7 OF INFLUENZA-VIRUS - COLINEAR AND INTERRUPTED MESSENGER-RNAS CODE FOR OVERLAPPING PROTEINS
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DOI：
10.1073/pnas.78.7.4170
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
CHOPPIN, PW
中科院分区：
文献类型：
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作者：
LAMB, RA;LAI, CJ;CHOPPIN, PW
甲型流感病毒基因组的 RNA 片段 7 编码至少 2 种蛋白质：M1 和 M2，它们是由不同的 mRNA 物种合成的。 M2 mRNA 的序列分析表明，它含有 689 个核苷酸的中断序列。 .apprx。包含M2 mRNA 5''末端前导序列的51个病毒特异性核苷酸与共线M1 mRNA 5''末端发现的相同。在 M2 mRNA 的前导序列之后，有一个 271 个核苷酸的主体区域，与 M1 mRNA 位于 3'' 共端。另一个与 RNA 片段 7 相关的小潜在 mRNA (mRNA3) 被发现。 mRNA3 的前导序列为 .apprx。 11 个病毒特异性核苷酸，与 M1 和 M2 mRNA 的 5'' 端相同，后面是 729 个核苷酸的中断序列，然后是 .apprx 的主体区域。 271个核苷酸与M2 mRNA相同。在M2 mRNA和mRNA3中中断序列的连接处发现的核苷酸序列与在真核mRNA中插入序列的剪接点处发现的核苷酸序列相似。两种 mRNA 的 5'' 末端均含有 10-15 个异源非病毒核苷酸，这些核苷酸似乎源自用于启动病毒 RNA 转录的细胞 RNA。由于 M1 mRNA 和 M2 mRNA 的 5'' 端序列相同，并且共享蛋白质合成的 5'' 端起始密码子，因此 M1 和 M2 蛋白质中的前 9 个氨基酸将相同，然后序列就会出现分歧。 .apprx。 M2 mRNA 的 271 个核苷酸的主体区域可以在 +1 阅读框内翻译，该序列表明 M1 和 M2 有 14 个氨基酸重叠。 mRNA3 的编码潜力仅为 9 个氨基酸，这些氨基酸与膜蛋白 (M1) 的 COOH 末端区域相同。
RNA segment 7 of the influenza A virus genome codes for at least 2 proteins, M1 and M2, which are synthesized from separate mRNA species. Sequence analysis of the M2 mRNA showed that it contains an interrupted sequence of 689 nucleotides. The .apprx. 51 virus-specific nucleotides comprising the 5''-end leader sequence of the M2 mRNA are the same as those found at the 5'' end of the colinear M1 mRNA. Following the leader sequence of the M2 mRNA, there is a 271-nucleotide body region that is 3'' coterminal with the M1 mRNA. Another small potential mRNA (mRNA3) related to RNA segment 7 was found. mRNA3 has a leader sequence of .apprx. 11 virus-specific nucleotides that are the same as the 5'' end of the M1 and M2 mRNA, followed by an interrupted sequence of 729 nucleotides and then a body region of .apprx. 271 nucleotides that is the same as the M2 mRNA. The nucleotide sequences found at the junctions of the interrupted sequences in M2 mRNA and mRNA3 are similar to those found at the splicing points of intervening sequences in eukaryotic mRNA. Both mRNA contain 10-15 heterogeneous nonviral nucleotides at their 5'' ends that appear to be derived from cellular RNA used for priming the transcription of viral RNA. Because the 5''-end sequences of the M1 mRNA and the M2 mRNA are the same and share the 5''-proximal initiation codon for protein synthesis, the first 9 amino acids would be the same in the M1 and M2 protein and then the sequences would diverge. The .apprx. 271-nucleotide body region of the M2 mRNA can be translated in the +1 reading frame and the sequence indicates that M1 and M2 overlap by 14 amino acids. The coding potential of the mRNA3 is for only 9 amino acids and these would be identical to the COOH-terminal region of the membrane protein (M1).