Brownian Dynamics Simulation Study on Force-velocity Relation in Actin-based Membrane Protrusion
Brownian Dynamics Simulation Study on Force-velocity Relation in Actin-based Membrane Protrusion
复制标题
肌动蛋白基膜突起力-速度关系的布朗动力学模拟研究
DOI：
10.1007/s40571-015-0051-x
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发表时间：
2015
影响因子：
3.3
通讯作者：
Taiji Adachi
中科院分区：
文献类型：
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作者：
Yasuhiro Inoue;Takeji Deji;Taiji Adachi
在基于肌动蛋白聚合的膜突出中，突出膜的速度随着相反力的增加而减小。这种关系称为力-速度关系 (FVR)，可以是上凹的，也可以是下凹的。然而，FVR 表现出两种趋势的机制仍存在争议。在这项研究中，我们模拟了由肌动蛋白聚合驱动的突出膜的布朗动力学。肌动蛋白扩散系数保持恒定，肌动蛋白聚合速率设置为低（case-L）或高（case-H）。案例 L 和案例 H 模拟中的突出膜分别表现出向上凹和向下凹的 FVR。聚合速率影响肌动蛋白的空间分布，从而影响它们与膜的物理接触。膜主要由案例 L 中的单体和案例 H 中的细丝支撑。这表明膜突起对相反力的反应不同，因为肌动蛋白在膜周围的分布不同。
In actin polymerization-based membrane protrusions, the velocity of the protruding membrane decreases with increasing opposing force. This relationship, known as the force–velocity relation (FVR), can be concave up or concave down. However, the mechanism by which FVR exhibits two trends remains debatable. In this study, we simulate the Brownian dynamics of the protruding membrane driven by actin polymerization. The actin diffusion coefficient is retained constant, and the actin polymerization rates are set to low (case-L) or high (case-H). The protruding membrane in the case-L and case-H simulations exhibits a concave up and concave down FVR, respectively. The polymerization rate affects the spatial distribution of actins, and thereby their physical contact with the membrane. The membrane is predominantly sustained by monomers in case-L and filaments in case-H. This suggests that membrane protrusions respond differently to the opposing force because actins are differently distributed around the membrane.