Association between MDM2-SNP309 and p53R72P polymorphisms and the risk of bladder cancer in the Mongolian population
Association between MDM2-SNP309 and p53R72P polymorphisms and the risk of bladder cancer in the Mongolian population
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DOI：
10.3892/mco.2017.1317
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发表时间：
2017-09-01
影响因子：
1.2
通讯作者：
Lee, Yi-Jang
中科院分区：
文献类型：
--
作者：
Avirmed, Shiirevnyamba;Wang, Bo-Shen;Lee, Yi-Jang
本研究旨在探讨MDM 2-SNP 309和p53 R72 P多态性是否与蒙古族人群膀胱癌的风险相关。这些多态性进行了评估，在79个对照组和63例膀胱癌病例使用PCR-限制性片段长度多态性分析，然后使用多变量logistic回归模型和Kaplan-Meier模型进行分析，以确定比值比（OR）和膀胱癌的发病年龄，分别。结果显示，MDM 2-SNP 309纯合（G/G）基因型与野生型（T/T）基因型相比，膀胱癌发病风险增加[OR=1.629; 95%可信区间（CI）=0.622-4.266]。另一方面，与野生型（R/R）基因型相比，p53 R72 P纯合（P/P）基因型倾向于保护人群免于膀胱癌（OR=0.445; 95%CI =0.1727-2.147）。MDM 2-SNP 309的GIG基因型与p53 R72 P的RIR基因型联合使用，可增加膀胱癌的发病风险（OR 3.355; 95% CI=0.3914-28.766）。按吸烟和慢性泌尿系统疾病史分层，MDM 2-SNP 309基因G/G（OR=2.3704; 95% CI=0.4308-3.044）和T/G（OR=5; 95% CI=0.8442-30.4088）基因型与膀胱癌的风险相关性增加。p53 R72 P的PIP的保护作用在分层后仍然存在。MDM 2-SNP 309和p53 R72 P与蒙古族膀胱癌的早期发病无关。MDM 2-SNP 309和p53 R72 P与蒙古族膀胱癌的发生无明显相关性。这两个SNP也不能预测膀胱癌发病的早期年龄。
The current study aims to investigate whether MDM2-SNP309 and p53R72P polymorphisms were associated with the risk of bladder cancer in Mongolian populations. These polymorphisms were evaluated in 79 controls and 63 bladder cancer cases using a PCR-restriction fragment length polymorphism assay, followed by analysis using multivariate logistic regression model and the Kaplan-Meier model to determine the odds ratio (OR) and age at onset of bladder cancer, respectively. The results revealed that the homozygous (G/G) genotype of MDM2-SNP309 increased the risk of bladder cancer compared to the wild-type (T/T) genotype [OR=1.629; 95% confidence interval (CI)=0.622-4.266] among Mongolians.On the other hand, the homozygous (P/P) genotype of p53R72P tended to protect the population from bladder cancer compared with the wild-type (R/R) genotype (OR=0.445; 95% CI=0.1727-2.147). It also showed that GIG genotype of MDM2-SNP309 increased the risk of bladder cancer when combined with the RIR genotype of p53R72P (OR 3.355; 95% CI=0.3914-28.766). Stratification by smoking and history of chronic urinary tract diseases tended towards increasing the risk association of the G/G (OR=2.3704; 95% CI=0.4308-3.044) and T/G genotypes (OR=5; 95% CI=0.8442-30.4088) of MDM2-SNP309 with bladder cancer, respectively. The protective role of PIP of p53R72P remained following stratification. MDM2-SNP309 and p53R72P were not involved in early age onset of bladder cancer in Mongolian patients. Taken together, MDM2-SNP309 and p53R72P had no significant association with bladder cancer in Mongolian patients. The two SNPs were also not able to predict early age at onset of bladder cancer.