Identification, isolation, and culture of intestinal epithelial stem cells from murine intestine.
Identification, isolation, and culture of intestinal epithelial stem cells from murine intestine.
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DOI：
10.1007/978-1-61779-815-3_6
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Magness ST
中科院分区：
文献类型：
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作者：
Gracz AD;Puthoff BJ;Magness ST
对成体干细胞群的研究提供了对正常生理和许多疾病状态下调节组织维持的机制的深入了解。肠道由多能干细胞库驱动，具有令人印象深刻的上皮更新率，是研究成体干细胞的特别有利的模型系统。直到最近，由于缺乏生物标志物和培养技术，肠上皮干细胞（IESC）的分离和体外研究仍然不可能。然而，IESC 分子表征和培养方面的进步使得大多数实验室都可以对这种细胞类型进行体外研究。本章描述的方法将使研究人员能够将新建立的技术应用于 IESC 的体外下游分析。
The study of adult stem cell populations provides insight into the mechanisms that regulate tissue maintenance in normal physiology and many disease states. With an impressive rate of epithelial renewal driven by a pool of multipotent stem cells, the intestine is a particularly advantageous model system for the study of adult stem cells. Until recently, the isolation and in vitro study of intestinal epithelial stem cells (IESCs) was not possible due to the lack of biomarkers and culture techniques. However, advances in molecular characterization and culture of IESCs have made in vitro studies on this cell type amenable to most laboratories. The methods described in this chapter will allow the investigator to adapt newly established techniques toward downstream analysis of IESCs in vitro.