Regulation of surface FcεRI expression an human eosinophils by IL-4 and IgE
Regulation of surface FcεRI expression an human eosinophils by IL-4 and IgE
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DOI：
10.1159/000053782
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发表时间：
2001-04-01
影响因子：
2.8
通讯作者：
Yamamoto, K
中科院分区：
文献类型：
--
作者：
Iikura, M;Yamaguchi, M;Yamamoto, K
背景资料：最近的研究表明，过敏性患者的嗜酸性粒细胞表面表达低水平的Fc ε RI，但嗜酸性粒细胞表面Fc ε RI表达的调控机制尚未完全了解。我们研究了IL-4和IgE是否能够影响正常人嗜酸性粒细胞表面Fc ε RI的表达，据报道，IL-4和IgE可调节人肥大细胞表面Fc ε RI的表达。研究方法：将从外周血中纯化的嗜酸性粒细胞与IL-5以及与或不与IL-4和/或IgE一起培养，并使用抗Fc β RI mAb CRA-1通过流式细胞术分析表面Fc β RI表达。结果如下：在用IL-4和IgE培养的嗜酸性粒细胞中观察到表观Fc ε RI表达（与肥大细胞Fc ε RI水平的1%相似）。IL-4（大于或等于1 ng/ml）和IgE（大于或等于0.5 μ g/ml）的组合对于最大程度诱导表面Fc γ RI表达是必需的。在IL-4和IgE的存在下，培养2天的嗜酸性粒细胞表现出低但具有统计学显著性的表面Fc β RI水平，其在培养7天后达到平台期。然而，CRA-1或抗IgE抗体交联表面Fc β RI分子不会诱导任何嗜酸性粒细胞活化。结论：IL-4和IgE可影响正常人嗜酸性粒细胞表面Fc受体的表达。嗜酸性粒细胞上的Fc ε RI表达可能受到与肥大细胞中类似的机制的调节。版权所有（C）2001 S. Karger AG，巴塞尔。
Background: Recent studies have demonstrated that eosinophils from allergic patients express low levels of Fc epsilon RI on their surface, but the regulatory mechanisms of eosinophil surface Fc epsilon RI expression are not fully understood. We investigated whether IL-4 and IgE, which are reported to regulate surface Fc epsilon RI expression on human mast cells, are able to affect surface Fc epsilon RI expression in normal human eosinophils. Methods: Eosinophils purified from peripheral blood were cultured with IL-5 and with or without IL-4 and/or IgE, and surface Fc epsilon RI expression was analyzed by flow cytometry using an anti-Fc epsilon RI mAb, CRA-1. Results: Apparent Fc epsilon RI expression (similar to 1% of mast cell Fc epsilon RI levels) was observed in eosinophils cultured with both IL-4 and IgE. A combination of IL-4 (greater than or equal to 1 ng/ml) and IgE (greater than or equal to 0.5 mug/ml) was necessary for the maximal induction of surface Fc epsilon RI expression. In the presence of IL-4 and IgE, eosinophils cultured for 2 days demonstrated low but statistically significant levels of surface Fc epsilon RI, which reached a plateau after 7 days of culture. However, cross-linkage of surface Fc epsilon RI molecules by CRA-1 or anti-IgE did not induce any eosinophil activation. Conclusions: IL-4 and IgE can affect the levels of surface Fc epsilon RI on normal human eosinophils. Fc epsilon RI expression on eosinophils may be regulated by a mechanism similar to that in mast cells. Copyright (C) 2001 S. Karger AG, Basel.