Detection of Diverse N-Acyl-Homoserine Lactones in Vibrio alginolyticus and Regulation of Biofilm Formation by N-(3-Oxodecanoyl) Homoserine Lactone In vitro.
Detection of Diverse N-Acyl-Homoserine Lactones in Vibrio alginolyticus and Regulation of Biofilm Formation by N-(3-Oxodecanoyl) Homoserine Lactone In vitro.
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溶藻弧菌中多种 N-酰基-高丝氨酸内酯的检测以及 N-(3-氧代癸酰基) 高丝氨酸内酯对生物膜形成的体外调节
DOI：
10.3389/fmicb.2017.01097
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发表时间：
2017
影响因子：
5.2
通讯作者：
Zhou L
中科院分区：
文献类型：
--
作者：
Liu J;Fu K;Wang Y;Wu C;Li F;Shi L;Ge Y;Zhou L
群体感应（Quorum sensing，QS）是一种细胞间的信息传递系统，它通过细胞间信号分子的交换实现，如N-酰基高丝氨酸内酯（N-Acyl homoserine lactones，AHLs），在大多数革兰氏阴性变形菌中，AHLs是QS基因表达的细胞密度调节因子，其种类和数量都有很大的差异。了解AHLs的调节可能有助于阐明细胞密度依赖性现象，如生物膜形成。溶藻弧菌是最常见的海洋条件弧菌病原体之一。然而，该物种的阿勒生产及其对生物膜形成的影响仍有待了解。在这里，我们的研究报告了47种海洋分离的溶藻弧菌菌株的不同阿勒谱以及外源3-oxo-C10-HSL在不同温度条件下（16°C和28°C）对生物膜形成的影响。共检测到11种AHLs，其中3-OH-C4-HSL、3-oxo-C10-HSL和3-oxo-C14-HSL所占比例最大。我们还观察到中等水平的外源3-oxo-C10-HSL（10和20 μM）可以诱导或增强溶藻弧菌生物膜的形成并改变其结构，而高水平（40和100 μM）没有显著改善甚至抑制生物膜的形成。此外，外源性3-氧代-C10-HSL的调节在溶藻弧菌中具有浓度和温度依赖性。
Quorum sensing (QS) is a cell-to-cell communication system based on the exchange of small intercellular signal molecules, such as N-Acyl homoserine lactones (AHLs), which act as cell-density mediators of QS gene expression, and are highly variable both in types and amounts in most Gram-negative Proteobacteria. Understanding the regulation of AHLs may contribute to the elucidation of cell density-dependent phenomena, such as biofilm formation. Vibrio alginolyticus is among the most frequently observed marine opportunistic Vibrio pathogens. However, AHL production of this species and its effects on biofilm formation remain to be understood. Here, our study reported the diverse AHL profiles of 47 marine-isolated V. alginolyticus strains and the effects of exogenous 3-oxo-C10-HSL on biofilm formation under different temperature conditions (16°C and 28°C). A total of 11 detected AHLs were produced by the isolates, of which 3-OH-C4-HSL, 3-oxo-C10-HSL and 3-oxo-C14-HSL comprised the largest proportions. We also observed that moderate levels of exogenous 3-oxo-C10-HSL (10 and 20 μM) could induce or enhance biofilm formation and alter its structure, while high levels (40 and 100 μM) did not significantly improve and even inhibited biofilm formation in V. alginolyticus. Further, regulation by exogenous 3-oxo-C10-HSL was both concentration- and temperature-dependent in V. alginolyticus.