Prediction of Epitope-Based Peptides for the Utility of Vaccine Development from Fusion and Glycoprotein of Nipah Virus Using In Silico Approach
Prediction of Epitope-Based Peptides for the Utility of Vaccine Development from Fusion and Glycoprotein of Nipah Virus Using In Silico Approach
复制标题
使用计算机模拟方法从尼帕病毒的融合和糖蛋白预测基于表位的肽在疫苗开发中的用途
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
A. Nabi
中科院分区：
文献类型：
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作者：
M. S. Sakib;M. Islam;A. M. Hasan;A. Nabi
本研究旨在通过针对尼帕病毒 (NiV) 的糖蛋白 G 和包膜蛋白 F 分别促进 NiV 与宿主细胞的附着和融合，设计用于疫苗开发的基于表位的肽。使用各种数据库和工具，测试了不同 NiV 分离株的 G 和 F 蛋白保守序列的免疫参数，以预测可能的表位。对肽与 MHC I 类和 II 类分子的结合分析、表位保守性、群体覆盖度和线性 B 细胞表位预测进行了分析。预测的肽与 7 个或更多 MHC 等位基因相互作用，并且 G 和 F 蛋白的群体覆盖率分别超过 99% 和 95%。预测的 I 类九聚体 SLIDTSSTI 和 EWISIVPNF 叠加在推定的十聚体 B 细胞表位上，也被鉴定为最可能的 II 类 15 聚体肽 GPKVSLIDTSSTITI 和 EWISIVPNFILVRNT 的核心序列。使用计算机对接技术进一步验证了这些肽与特定 HLA 等位基因的结合。我们的计算机分析表明，预测的表位 GPKVSLIDTSSTITI 或 EWISIVPNFILVRNT 可能是作为针对 NiV 的通用疫苗成分的更好选择，无论不同的分离株都可能引发体液和细胞介导的免疫。
This study aims to design epitope-based peptides for the utility of vaccine development by targeting glycoprotein G and envelope protein F of Nipah virus (NiV) that, respectively, facilitate attachment and fusion of NiV with host cells. Using various databases and tools, immune parameters of conserved sequence(s) from G and F proteins of different isolates of NiV were tested to predict probable epitope(s). Binding analyses of the peptides with MHC class-I and class-II molecules, epitope conservancy, population coverage, and linear B cell epitope prediction were analyzed. Predicted peptides interacted with seven or more MHC alleles and illustrated population coverage of more than 99% and 95%, for G and F proteins, respectively. The predicted class-I nonamers, SLIDTSSTI and EWISIVPNF, superimposed on the putative decameric B cell epitopes, were also identified as core sequences of the most probable class-II 15-mer peptides GPKVSLIDTSSTITI and EWISIVPNFILVRNT. These peptides were further validated for their binding to specific HLA alleles using in silico docking technique. Our in silico analysis suggested that the predicted epitopes, either GPKVSLIDTSSTITI or EWISIVPNFILVRNT, could be a better choice as universal vaccine component against NiV irrespective of different isolates which may elicit both humoral and cell-mediated immunity.
影响因子：
56.9
作者：
HUNT, DF;HENDERSON, RA;ENGELHARD, VH
通讯作者：
ENGELHARD, VH