Synthetic peptide fragment of src gene product inhibits the src protein kinase and crossreacts immunologically with avian onc kinases and cellular phosphoproteins.
Synthetic peptide fragment of src gene product inhibits the src protein kinase and crossreacts immunologically with avian onc kinases and cellular phosphoproteins.
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src 基因产物的合成肽片段抑制 src 蛋白激酶并与禽类 onc 激酶和细胞磷蛋白发生免疫交叉反应。
DOI：
10.1073/pnas.78.12.7412
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发表时间：
1981
影响因子：
11.1
通讯作者：
Goldberg,AR
中科院分区：
文献类型：
--
作者：
Wong,TW;Goldberg,AR
所有已知的禽肉瘤病毒都具有相关的蛋白激酶活性，可磷酸化其靶蛋白的酪氨酸残基。劳斯肉瘤病毒 (RSV) pp60src 的十肽片段、残基 415-424 以及该序列的类似物已通过固相方法化学合成。这两种十肽不被RSV的pp60src、Y73禽肉瘤病毒的P90或富士南肉瘤病毒的P140磷酸化。然而，两种肽都能够竞争性抑制与转化蛋白相关的激酶活性。针对其中一种肽产生抗血清，并通过亲和层析从血清中纯化 IgG。该抗体能够从感染 RSV 的细胞中沉淀出 RSV 的 pp60src 以及 Y73 病毒的 P90。该抗体还从正常鸡和大鼠成纤维细胞以及多个病毒转化细胞系中沉淀出许多高分子量磷蛋白。
All the known avian sarcoma viruses have associated protein kinase activities that phosphorylate tyrosine residues of their target proteins. A decapeptide fragment of pp60src of Rous sarcoma virus (RSV), residues 415-424, and an analog of that sequence have been chemically synthesized by solid-phase methods. The two decapeptides were not phosphorylated by pp60src of RSV, P90 of Y73 avian sarcoma virus, or P140 of Fujinami sarcoma virus. However, both peptides were able to inhibit competitively the kinase activities associated with the transforming proteins. Antiserum was raised against one of the peptides and IgG was purified from the serum by affinity chromatography. The antibody was able to precipitate pp60src of RSV as well as P90 of Y73 virus from cells infected with these viruses. The antibody also precipitated a number of high molecular weight phosphoproteins from normal chicken and rat fibroblasts and from several lines of virus-transformed cells.