Nucleo-cytoplasmic Partitioning of ARF Proteins Controls Auxin Responses in Arabidopsis thaliana
Nucleo-cytoplasmic Partitioning of ARF Proteins Controls Auxin Responses in Arabidopsis thaliana
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DOI：
10.1016/j.molcel.2019.06.044
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发表时间：
2019-10-03
期刊：
影响因子：
16
通讯作者：
Strader, Lucia C.
中科院分区：
文献类型：
--
作者：
Powers, Samantha K.;Holehouse, Alex S.;Strader, Lucia C.
植物激素生长素在植物生长和发育的几乎每个方面都起着至关重要的作用。生长素反应因子（ARF）转录因子家族调节生长素反应基因的表达，并在高生长素反应区域表现出核定位。在这里，我们表明，ARF 7和ARF 19蛋白积累在细胞质内的生长素反应减弱的组织微米大小的组件。我们发现ARF的内在无序的中间区域和折叠的PB 1相互作用结构域驱动蛋白质组装形成。PB 1结构域内单个赖氨酸的突变消除了细胞质组装，促进了ARF核定位，并导致转录组改变和形态缺陷。我们的数据表明，ARF核-细胞-细胞质分配调节生长素的反应，提供了一种机制，细胞的能力生长素信号。
The phytohormone auxin plays crucial roles in nearly every aspect of plant growth and development. The auxin response factor (ARF) transcription factor family regulates auxin-responsive gene expression and exhibits nuclear localization in regions of high auxin responsiveness. Here we show that the ARF7 and ARF19 proteins accumulate in micron-sized assemblies within the cytoplasm of tissues with attenuated auxin responsiveness. We found that the intrinsically disordered middle region and the folded PB1 interaction domain of ARFs drive protein assembly formation. Mutation of a single lysine within the PB1 domain abrogates cytoplasmic assemblies, promotes ARF nuclear localization, and results in an altered transcriptome and morphological defects. Our data suggest a model in which ARF nucleo-cyto-plasmic partitioning regulates auxin responsiveness, providing a mechanism for cellular competence for auxin signaling.