Human Endometrial Mesenchymal Stem Cells Modulate the Tissue Response and Mechanical Behavior of Polyamide Mesh Implants for Pelvic Organ Prolapse Repair
Human Endometrial Mesenchymal Stem Cells Modulate the Tissue Response and Mechanical Behavior of Polyamide Mesh Implants for Pelvic Organ Prolapse Repair
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DOI：
10.1089/ten.tea.2013.0170
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发表时间：
2014-02-01
影响因子：
4.1
通讯作者：
Gargett, Caroline E.
中科院分区：
文献类型：
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作者：
Ulrich, Daniela;Edwards, Sharon Lee;Gargett, Caroline E.
背景：盆腔器官脱垂（POP）是指一个或多个盆腔结构下降到阴道内，包括子宫、阴道穹隆和阴道前壁或后壁脱垂。POP的治疗可包括植入合成补片。然而，由于补片暴露或疼痛等并发症，补片手术的长期受益存在争议。本研究的目的是使用组织工程（TE）的方法，以评估thein的体内生物学和生物力学行为的一种新的明胶/聚酰胺网，接种了一种新的来源的间充质干细胞在皮下大鼠模型的伤口repair.Methods：W5 C5富集的人子宫内膜间充质干细胞（eMSC）接种到网格（明胶涂层聚酰胺针织）在100，000细胞/cm 2。将含或不含细胞的补片皮下背侧植入免疫功能低下大鼠中7、30、60和90天。流式细胞仪检测DiO标记细胞的增殖情况。进行异物反应和组织整合的免疫组织化学评估。测定总胶原和III型和I型胶原的水平。结果：植入后第7天和第90天，移植物的耐受性良好，在移植物上可检测到标记细胞。具有细胞的网状物在第7天促进显著更多的新血管形成（p<0.05），并且在第90天吸引更少的巨噬细胞（p<0.05）。同样，在90天时，接种细胞的补片中的白细胞浸润显著较低（p<0.05）。与细胞的网格一般不太僵硬比那些没有细胞，7和90 days implantation.Conclusion：TE的方法在这项研究中使用的显着减少周围的炎症细胞的数量，并促进新血管形成。接种eMSC发挥抗炎作用，促进伤口修复，新组织生长和最小纤维化，并产生具有更大延展性的补片。将细胞接种到聚酰胺/明胶补片上可改善补片的生物相容性，并可能成为未来治疗POP的替代选择。
Background:Pelvic organ prolapse (POP) is defined as the descent of one or more of the pelvic structures into the vagina and includes uterine, vaginal vault, and anterior or posterior vaginal wall prolapse. The treatment of POP may include implantation of a synthetic mesh. However, the long-term benefit of mesh surgery is controversial due to complications such as mesh exposure or pain. The aim of this study was to use a tissue engineering (TE) approach to assess thein vivobiological and biomechanical behavior of a new gelatin/polyamide mesh, seeded with a novel source of mesenchymal stem cells in a subcutaneous rat model of wound repair.Methods:W5C5-enriched human endometrial mesenchymal stem cells (eMSC) were seeded onto meshes (gelatin-coated polyamide knit) at 100,000 cells/cm2. Meshes, with or without cells were subcutaneously implanted dorsally in immunocompromised rats for 7, 30, 60, and 90 days. Flow cytometry was used to detect DiO labeled cells after explantation. Immunohistochemical assessment of foreign body reaction and tissue integration were conducted. Total collagen and the levels of collagens type III and type I were determined. Uniaxial tensiometry was performed on explanted meshes, originally seeded with and without cells, at days 7 and 90.Results:Implanted meshes were well tolerated, with labeled cells detected on the mesh up to 14 days postimplantation. Meshes with cells promoted significantly more neovascularization at 7 days (p<0.05) and attracted fewer macrophages at 90 days (p<0.05). Similarly, leukocyte infiltration was significantly lower in the cell-seeded meshes at 90 days (p<0.05). Meshes with cells were generally less stiff than those without cells, after 7 and 90 days implantation.Conclusion:The TE approach used in this study significantly reduced the number of inflammatory cells around the implanted mesh and promoted neovascularization. Seeding with eMSC exerts an anti-inflammatory effect and promotes wound repair with new tissue growth and minimal fibrosis, and produces mesh with greater extensibility. Cell seeding onto polyamide/gelatin mesh improves mesh biocompatibility and may be an alternative option for future treatment of POP.