Autoradiographic analyses of the effects of restraint-induced stress on 5-HT1A, 5-HT1C and 5-HT2 receptors in the dorsal hippocampus of male and female rats.
Autoradiographic analyses of the effects of restraint-induced stress on 5-HT1A, 5-HT1C and 5-HT2 receptors in the dorsal hippocampus of male and female rats.
复制标题
放射自显影分析约束诱导的应激对雄性和雌性大鼠背侧海马 5-HT1A、5-HT1C 和 5-HT2 受体的影响。
DOI：
10.1159/000125951
复制
发表时间：
1991
影响因子：
4.1
通讯作者：
McEwen,BS
中科院分区：
文献类型：
--
作者：
Mendelson,SD;McEwen,BS
用定量放射自显影法评价性别和每天1次或5次2小时束缚应激对大鼠背侧海马5-HT 1A、5-HT 1C和5-HT 2受体结合的影响。性别和束缚应激均未发现对5-HT 1或5-HT 2受体的结合有影响。然而，束缚应激增加了[3 H]8-羟基-2-（二正丙基氨基）四氢萘在CA 4区和锥体下齿状回的5-HT 1A受体的结合。此外，在5-HT 1A受体的结合水平在CA 1区的oriens和腔隙分子层显着较高的雌性大鼠。苯甲酸雌二醇或苯甲酸雌二醇加孕酮对卵巢切除大鼠海马5-HTIA受体的结合均无影响，这使得性别差异不太可能与发情周期的阶段有关。应激诱导的皮质酮（CORT）水平较高的女性。尽管雄性和雌性大鼠在束缚期间获得的血液中的CORT水平从第1次会议到第5次会议均有所下降，但仅雌性大鼠的下降幅度显著。雌性大鼠在开阔场地也表现出更高水平的活动。尽管束缚后雄性和雌性大鼠在旷场的活动减少，但这些减少并不显著。结果进行了讨论有关焦虑和抑郁。
Quantitative autoradiography was used to evaluate the effects of sex and either 1 or 5 daily 2-hour sessions of restraint stress on binding at 5-HT1A, 5-HT1C and 5-HT2receptors in the rat dorsal hippocampus. Neither sex nor restraint stress were found to have effects on binding at 5-HT1cor 5-HT2receptors. However, restraint stress increased binding of [3H]8-hydroxy-2-(di-n-propylamino)tetralin at 5-HT1Areceptors in the CA4 region and in the infrapyramidal dentate gyrus. In addition, levels of binding at 5-HT1Areceptors in the oriens and lacunosum moleculare layers of the CA1 region were significantly higher in female rats. Neither estradiol benzoate nor estradiol benzoate plus progesterone had effects on binding at hippocampal 5-HTIA receptors in ovariectomized rats, making it unlikely that the sex differences were related to stages of the estrous cycle. Stress-induced levels of corticosterone (CORT) were higher in females. Although CORT levels in blood obtained during restraint decreased from session 1 to session 5 in both male and female rats, the decrease became significant in females only. Female rats also displayed higher levels of activity in the open field. Although activity in the open field was reduced in male and female rats after restraint, these decreases were not significant. Results are discussed in relation to anxiety and depression.