Separation of water-soluble polysaccharides from Cyclocarya paliurus by ultrafiltration process
Separation of water-soluble polysaccharides from Cyclocarya paliurus by ultrafiltration process
复制标题
超滤法分离青钱柳水溶性多糖
DOI：
10.1016/j.carbpol.2013.09.075
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发表时间：
2014-01-30
影响因子：
11.2
通讯作者：
Xie, Ming-Yong
中科院分区：
文献类型：
--
作者：
Xie, Jian-Hua;Shen, Ming-Yue;Xie, Ming-Yong
采用超滤膜法分离青钱柳多糖。Iljinskaja（C. paliurus）以模拟工业生产。同时，对C.采用凝胶渗透色谱法对马蹄莲多糖进行了研究。共得到4个组分，分别命名为CPPS-A、CPPS-B、CPPS-C和CPPS-D。CPPS-A和CPPS-B分别含有约69.5%和12.7%的多糖，其分子量在100-300 kDa和120 kDa的范围内。CPPS-C由平均分子量为40 kDa和15 kDa的两种多糖组成。结果表明，超滤可去除部分小分子多糖，提高大分子多糖的比例，产品质量明显提高。与乙醇沉淀法和凝胶渗透色谱法相比，超滤法显示出许多优点，为工业化生产香菇提供了理论支持。分离出的马蹄莲多糖。(C)2013爱思唯尔有限公司保留所有权利。
In this study, ultrafiltration membrane process was employed to separate polysaccharides from Cyclocarya paliurus (Batal.) Iljinskaja (C. paliurus) to simulate industrial production. Meanwhile, the molecular weight distribution of C. paliurus polysaccharides was investigated by gel permeation chromatography. Four fractions were obtained and named as CPPS-A, CPPS-B, CPPS-C and CPPS-D, respectively. CPPS-A and CPPS-B contained approximately 69.5% and 12.7% of polysaccharides, whose molecular weight were in the range of 100-300 kDa and 120 kDa, respectively. CPPS-C was comprised of two polysaccharides with average molecular weight of 40 kDa and 15 kDa. Results showed that ultrafiltration resulted in the removal of parts of small molecule weight polysaccharides, the increase of proportion of high molecule weight ones and the obvious improvement of quality of products. Compared with ethanol precipitation and gel permeation chromatography techniques, ultrafiltration showed many advantages, and also provided theoretical support for industrial manufacturing of C. paliurus polysaccharides in separation. (C) 2013 Elsevier Ltd. All rights reserved.