An Additive Manufacturing Approach that Enables the Field Deployment of Synthetic Biosensors
An Additive Manufacturing Approach that Enables the Field Deployment of Synthetic Biosensors
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DOI：
10.1016/j.eng.2018.12.001
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发表时间：
2019-03
期刊：
影响因子：
12.8
通讯作者：
Daniel Wolozny;J. R. Lake;Paul G. Movizzo;Zhicheng Long;Warren C. Ruder
中科院分区：
文献类型：
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作者：
Daniel Wolozny;J. R. Lake;Paul G. Movizzo;Zhicheng Long;Warren C. Ruder
合成生物学工具可用于设计报告分析物存在的活体生物传感器。尽管这些工程细胞生物传感器在实验室外有许多潜在的应用，但它们是转基因生物（GMO），通常被认为是危险的。降低将转基因生物释放到环境中的风险，同时使其能够在实验室外使用至关重要。在这里，我们描述了一种由合成生物电路组成的生物传感系统的开发，该系统是在大肠杆菌中设计的，包含在独特的3D打印设备外壳中。这些转基因生物检测铜绿假单胞菌的化学群体信号，铜绿假单胞菌是一种机会致病菌。使用该装置，活体生物传感器与感兴趣的样本接触，而不会暴露于环境中。细胞可以在培养管中进行现场目视分析，或返回实验室进行进一步分析。许多生物传感器缺乏现场部署所需的多功能性，由于缺乏资源和设备，许多疾病可能无法诊断。我们的生物测定设备利用3D打印来创建便携式，模块化和廉价的设备，用于现场部署活体生物传感器。
The tools of synthetic biology can be used to engineer living biosensors that report the presence of analytes. Although these engineered cellular biosensors have many potential applications for deployment outside of the lab, they are genetically modified organisms (GMOs) and are often considered dangerous. Mitigating the risk of releasing GMOs into the environment while enabling their use outside a laboratory is critical. Here, we describe the development of a biosensing system consisting of a synthetic biological circuit, which is engineered inEscherichia colithat are contained within a unique 3D-printed device housing. These GMOs detect the chemical quorum signal ofPseudomonas aeruginosa, an opportunistic pathogen. Using this device, the living biosensor makes contact with a specimen of interest without ever being exposed to the environment. Cells can be visually analyzed in the field within culture tubes, or returned to the lab for further analysis. Many biosensors lack the versatility required for deployment in the field, where many diseases can go undiagnosed due to a lack of resources and equipment. Our bioassay device utilizes 3D printing to create a portable, modular, and inexpensive device for the field deployment of living biosensors.