Establishment of various biliary tract carcinoma cell lines and xenograft models for appropriate preclinical studies.
Establishment of various biliary tract carcinoma cell lines and xenograft models for appropriate preclinical studies.
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建立各种胆道癌细胞系和异种移植模型，用于适当的临床前研究。
DOI：
10.3748/wjg.v22.i40.9035
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发表时间：
2016-10-28
影响因子：
4.3
通讯作者：
Shibata T
中科院分区：
文献类型：
--
作者：
Ojima H;Yamagishi S;Shimada K;Shibata T
我们最近报道了几个胆道癌(BTC)的驱动基因，已知在肿瘤发生和疾病进展中发挥重要作用。尽管开发新的治疗策略的需求正在增加，但目前可用于进行临床前研究的BTC细胞系和异种移植模型很少。利用88例BTC手术标本和536只免疫缺陷小鼠，建立了28个BTC异种移植模型和13个新的BTC细胞系，包括亚型。我们的一些细胞株被发现对吉西他滨具有耐药性，吉西他滨目前是首选治疗方法，从而使高度实用的临床前研究得以进行。利用上述细胞系和异种移植模型以及BTC切除患者的临床病理数据库，我们可以建立一个临床前研究体系和合适的药物疗效研究参数，以探索新的生物标志物在未来研究中的实际应用。
We recently reported several driver genes of biliary tract carcinoma (BTC) that are known to play important roles in oncogenesis and disease progression. Although the need for developing novel therapeutic strategies is increasing, there are very few BTC cell lines and xenograft models currently available for conducting preclinical studies. Using a total of 88 surgical BTC specimens and 536 immunodeficient mice, 28 xenograft models and 13 new BTC cell lines, including subtypes, were established. Some of our cell lines were found to be resistant to gemcitabine, which is currently the first choice of treatment, thereby allowing highly practical preclinical studies to be conducted. Using the aforementioned cell lines and xenograft models and a clinical pathological database of patients undergoing BTC resection, we can establish a preclinical study system and appropriate parameters for drug efficacy studies to explore new biomarkers for practical applications in the future studies.
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影响因子：
3.3
作者：
Morofuji, Noriaki;Ojima, Hidenori;Kondo, Tadashi
通讯作者：
Kondo, Tadashi
影响因子：
2.4
作者：
Takahashi, Hiroyuki;Ojima, Hidenori;Shibata, Tatsuhiro
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Shibata, Tatsuhiro
影响因子：
5.7
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5.7
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通讯作者：
Shibata, Tatsuhiro
影响因子：
30.8
作者：
Nakamura, Hiromi;Arai, Yasuhito;Shibata, Tatsuhiro
通讯作者：
Shibata, Tatsuhiro