Immobilized enzyme carbon paste electrodes as amperometric sensors
Immobilized enzyme carbon paste electrodes as amperometric sensors
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作为电流传感器的固定化酶碳糊电极
DOI：
10.1002/elan.1140030412
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发表时间：
1991
期刊：
影响因子：
3
通讯作者：
L. Hernandez
中科院分区：
文献类型：
--
作者：
E. Lorenzo;E. González;F. Pariente;L. Hernandez
介绍了新型碳糊酶电极的结构和性能。黄嘌呤氧化酶和碱性磷酸酶被吸附在碳糊电极（CPE）上，并被半透膜物理包埋。酶促反应的产物尿酸或对硝基苯酚分别在+0.4或+0.8 V下对Ag/AgCl电极进行电化学氧化，产生与底物浓度直接相关的稳态电流。响应特性以及动力学参数进行了评价。
The construction and performance of new carbon paste enzyme electrodes as amperometric sensors is described. Xanthine oxidase and alkaline phosphatase have been adsorbed on a carbon paste electrode (CPE), and physically entrapped with a semipermeable membrane. The product of enzymatic reaction, uric acid or p-nitrophenol, was electrochemically oxidized versus an Ag/AgCl electrode at +0.4 or +0.8 V, respectively, yielding a steady-state current directly related to the substrate concentration. Response characteristics as well as kinetic parameters have been evaluated.