Thymic stroma-derived T cell growth factor (TSTGF). I. Functional distinction of TSTGF from interleukins 2 and 4 and its preferential growth-promoting effect on helper T cell clones.
Thymic stroma-derived T cell growth factor (TSTGF). I. Functional distinction of TSTGF from interleukins 2 and 4 and its preferential growth-promoting effect on helper T cell clones.
复制标题
胸腺基质衍生的 T 细胞生长因子 (TSTGF)。
作者：
M. Ogata;Soichiro Sato;H. Sano;Toshiyuki Hamaoka;Hideyuki Doi;Kenji Nakanishi;Yoshihiro Asano;Tsunetoshi Itoh;H. Fujiwara
最近建立的胸腺基质来源细胞系(TSCL)支持白介素2(IL)2依赖的抗原特异性辅助性T细胞(Th)克隆9-16的生长，而不需要IL-2和抗原，这种生长被该TSCL产生的培养因子所取代。胸腺基质衍生T细胞生长因子(TSTGF)能诱导包括9-16 Th克隆在内的多种Th克隆的增殖，但不能诱导细胞毒性T细胞克隆的增殖。TSTGF诱导的生长促进作用以剂量依赖的方式获得，并维持Th克隆的抗原特异性。TSCL的培养上清不含IL-1、IL-2、IL-3、IL-4或干扰素活性。重组IL-2、IL-4和TSTGF均能刺激9-16 Th克隆的增殖，而IL-1、IL-3和干扰素则不能刺激其增殖。然而，IL-2或IL-4对Th克隆的增殖几乎完全被抗IL-2受体或抗IL-4单抗所抑制，而TSTGF诱导的9-16 Th克隆的生长不受这两种抗体的影响，说明TSTGF在功能上不同于IL-2和IL-4。此外，从新鲜制备的原代胸腺外植体的培养上清液中也检测到了TSTGF活性。这些结果表明，原始胸腺外植体和已建立的TSCL在缺乏T细胞生长因子的情况下，能够促进辅助型T细胞的生长，但不能促进细胞毒型T细胞的生长。
A recently established thymic stroma-derived cell line (TSCL) supported the growth of the interleukin (IL) 2-dependent, antigen-specific helper T cell (Th) clone, 9-16, without requirement for IL-2 and antigen, and such growth was substituted by a factor produced into cultures by this established TSCL. This substance, thymic stroma-derived T cell-growth factor (TSTGF), was capable of inducing the proliferation of various Th clones including 9-16 Th clone, but not of cytotoxic T cell clones. TSTGF-induced growth promotion was obtained in a dose-dependent fashion and in maintaining antigen specificity of Th clones. The culture supernatant from the TSCL did not contain detectable level of IL-1, IL-2, IL-3, IL-4, or interferon activity. The proliferation of 9-16 Th clone was stimulated by recombinant IL-2 and IL-4 as well as TSTGF, but not by IL-1, IL-3, or interferons. However, the proliferation of this Th clone by IL-2 or IL-4 was almost completely inhibited by anti-IL-2 receptor or anti-IL-4 monoclonal antibody, respectively, whereas TSTGF-induced growth of 9-16 Th clones was not affected by either type of antibody, demonstrating that TSTGF is functionally distinct from IL-2 and IL-4. In addition, TSTGF activity was also obtained from the culture supernatant of the primary thymic explant, which was freshly prepared. These results indicate that the primary thymic explant as well as an established TSCL produce factors capable of promoting the growth of helper but not cytotoxic type of T cells in the absence of T cell growth factors thus far defined.