Six Schizosaccharomyces pombe tRNA genes including a gene for a tRNALys with an intervening sequence which cannot base-pair with the anticodon.
Six Schizosaccharomyces pombe tRNA genes including a gene for a tRNALys with an intervening sequence which cannot base-pair with the anticodon.
复制标题
六个粟酒裂殖酵母 tRNA 基因，包括一个 tRNALys 基因，其间插序列不能与反密码子碱基配对。
作者：
V. Gamulin;J. Mao;B. Appel;M. Sumner;F. Yamao;D. Söll
我们报道了六个S的序列。pombe tRNA基因包括两个tRNAArg基因和tRNAGlu、tRNAHis、tRNALys和tRNAPhe各一个基因。所有tRNA基因都独立地存在于基因组中，并代表单独的转录单位。tRNALys基因有一个8 bp长的插入序列，不能与tRNA反密码子碱基配对。体外转录研究表明，所有基因在酵母提取物中都能忠实地转录。tRNA基因的5'侧翼区的序列比较没有显示出显著的同源性;然而，它们非常富含AT碱基对。
We report the sequences of six S. pombe tRNA genes including two genes for tRNAArg, and one gene each for tRNAGlu, tRNAHis, tRNALys and tRNAPhe. All tRNA genes are found independently in the genome and represent individual transcription units. The gene for tRNALys has an 8 bp long intervening sequence which cannot base-pair with the tRNA anticodon. In vitro transcription studies indicate that all genes are faithfully transcribed in a yeast extract. Sequence comparison of the 5' flanking regions of the tRNA genes did not show significant homologies; however, they are very rich in AT base pairs.