Multi-walled carbon nanotubes as solid-phase extraction sorbents for simultaneous determination of type A trichothecenes in maize, wheat and rice by ultra-high performance liquid chromatography-tandem mass spectrometry
Multi-walled carbon nanotubes as solid-phase extraction sorbents for simultaneous determination of type A trichothecenes in maize, wheat and rice by ultra-high performance liquid chromatography-tandem mass spectrometry
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多壁碳纳米管固相萃取吸附剂超高效液相色谱-串联质谱同时测定玉米、小麦和水稻中的A型单端孢霉烯族化合物
DOI：
10.1016/j.chroma.2015.10.068
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发表时间：
2015-12-04
影响因子：
4.1
通讯作者：
Han, Zheng
中科院分区：
文献类型：
--
作者：
Dong, Maofeng;Si, Wenshuai;Han, Zheng
建立了以多壁碳纳米管（MWCNTs）为吸附剂的固相萃取（SPE）-超高效液相色谱-串联质谱（UHPLC-MS/MS）同时测定玉米、小麦和水稻中4种A型多烯类抗生素的方法。包括样品上样溶液的组成，洗涤和洗脱溶剂的几个关键参数进行了彻底的研究，以实现最佳的SPE回收率和效率。pH值对MWCNTs材料的性能有显著影响，优化后，以正己烷和5%甲醇水溶液为洗脱液，以含1%甲酸的甲醇为洗脱溶剂，对不同基质中的4种目标物均表现出良好的净化效果。通过测定线性（R-2 >= 0.992）、回收率（73.4-113.7%）、精密度（1.2-17.1%）和灵敏度（定量限在0.02-0.10 μ g/kg范围内）对该方法进行了验证，并进一步应用于30份样品中A型单端孢霉烯的同时测定。尽管观察到小麦、玉米和水稻中A型单端孢霉烯的污染水平较低，这表明中国上海消费者的风险有所降低，但开发的方法已被证明是复杂作物基质中A型单端孢霉烯监测的宝贵工具。(C)2015 Elsevier B. V.版权所有。
A solid-phase extraction (SPE) procedure using multi-walled carbon nanotubes (MWCNTs) as sorbents coupled with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed for simultaneous determination of four type A trichothecenes in maize, wheat and rice for the first time. Several key parameters including the composition of sample loading solutions, washing and elution solvents were thoroughly investigated to achieve optimal SPE recoveries and efficiency. Performance of the MWCNTs materials was significantly affected by pH, and after optimization, n-hexane and 5% methanol aqueous solution as the washing solutions and methanol containing 1% formic acid as the elution solvent presented an excellent purification efficiency for the four targets in the different matrices. The method was validated by determining the linearity (R-2 >= 0.992), recovery (73.4-113.7%), precision (1.2-17.1%) and sensitivity (limit of quantification in the range of 0.02-0.10 mu g/kg), and was further applied for simultaneous determination of type A trichothecenes in 30 samples. Although low contamination levels of type A trichothecenes in wheat, maize and rice were observed revealing mitigated risks to consumers in Shanghai, China, the developed method has proven to be a valuable tool for type A trichothecenes monitoring in complex crop matrices. (C) 2015 Elsevier B.V. All rights reserved.