Fosinopril and valsartan intervention in gene expression of Klotho, MMP-9, TIMP-1, and PAI-1 in the kidney of spontaneously hypertensive rats.
Fosinopril and valsartan intervention in gene expression of Klotho, MMP-9, TIMP-1, and PAI-1 in the kidney of spontaneously hypertensive rats.
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DOI：
10.3969/j.issn.1672-7347.2010.10.004
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发表时间：
2010-10-01
期刊：
影响因子：
--
通讯作者：
Wu, Rong
中科院分区：
文献类型：
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作者：
Cheng, Xiaomiao;Zhou, Qiaoling;Wu, Rong
目的：探讨福辛普利和缬沙坦干预对自发性高血压大鼠(SHR)肾组织Klotho、基质金属蛋白酶-9(MMP-9)、组织金属蛋白酶抑制物-1(TIMP-1)和纤溶酶原激活物抑制物-1(PAI-1)基因表达的影响。方法：将20只22周龄雄性SHR随机分为4组：高血压组(SHR组)、福辛普利组[Fos组，10 mg/(kg.d)灌胃]、valsartan组[Val组，每组5只]。福辛普利+缬沙坦组[Fos+Val组，福辛普利10 mg/(kg·d)+valsartan 50 mg/(kg·d)组]。另取5只22周龄雄性Wistar京都大鼠(WKY)作为对照。实验8周后，通过监测大鼠体重、尾动脉压、福辛普利和/或valsartan干预后24小时尿蛋白定量。结果：逆转录聚合酶链式反应(RT-PCR)显示SHR大鼠肾脏Klotho基因及蛋白表达显著降低(P
OBJECTIVE: To determine the role of fosinopril and valsartan intervention in Klotho, matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1) and plasminogen activator inhibitor (PAI-1) gene expression in hypertensive renal interstitial fibrosis (RIF) in the kidney tissue of spontaneously hypertensive rats (SHR).METHODS: We randomly divided 20 male 22-week-old SHR into 4 groups (5 in each group): a hypertension group (SHR group), a fosinopril group [Fos group, 10 mg/( kg · d) gavage], a valsartan group [Val group, 10 mg/( kg · d) gavage], and a fosinopril plus valsartan group [Fos + Val group, fosinopril 10 mg/( kg · d) + valsartan 50 mg/( kg · d) gavage]. Another five 22-week-old male Wistar Kyoto rats (WKY) were used as controls. Through monitoring the weight of the rats, tail artery pressure, 24-hour urine protein by fosinopril and/or valsartan intervention after the 8-week trial. RT-PCR and Western blot were used to detect the mRNA and protein expression of Klotho, MMP-9, TIMP-1, and PAI-1 in the kidneys.RESULTS: RT-PCR showed that in the SHR group, Klotho mRNA and protein expression were significantly decreased(P