TIME-RESOLVED SPECTROSCOPY OF HEMOGLOBIN AND MYOGLOBIN IN RESTING AND ISCHEMIC MUSCLE
TIME-RESOLVED SPECTROSCOPY OF HEMOGLOBIN AND MYOGLOBIN IN RESTING AND ISCHEMIC MUSCLE
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DOI：
10.1016/0003-2697(88)90076-0
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发表时间：
1988-11-01
影响因子：
2.9
通讯作者：
HOLTOM, G
中科院分区：
文献类型：
--
作者：
CHANCE, B;NIOKA, S;HOLTOM, G
肌肉中血红蛋白/肌红蛋白吸收变化的量化困难导致了使用短脉冲光的新方法的发展。这种方法使用足够短的输入光脉冲，因此可以精确地测量光通过大脑传播的时间进程。给定光在组织中的速度，光到达探测器的时间就给出了光路长度。光子在组织中迁移的强度分布允许确定激发光子经过的路径长度和色素的浓度变化。腔倾倒液体染料激光用130-ps脉冲照射组织，检测到在距输入点3.0厘米处的一半高度处持续600-ps脉冲。从50%点到0.1%的强度衰减遵循斜率的对数函数。这归因于组织的总吸收系数。增量.MU。由于脱氧血红蛋白在760和630 nm处的吸收被用来计算浓度变化。这允许针对特定几何图形计算2厘米的连续光测量的路径长度。激光波长的变化提供了对组织变化光谱的确定。
Difficulties of quantitation of hemoglobin/myoglobin absorption changes in muscle have led to the development of a new approach using short pulses of light. This method uses input light pulses sufficiently short so that the time course of travel of light through the brain can be precisely measured. The time of arrival of light at the detector gives the optical path length, given the velocity of light in tissues. The intensity profile of photon migration in tissues permits determination of the path length that the exiting photons have traveled and the concentration change of the pigments. A cavity-dumped liquid dye laser illuminates the tissue with 130-ps pulses detected as 600-ps duration at a half height at 3.0-cm distance from the input point. The decay of intensity from the 50% point onward to 0.1% follows a logarithmic function of slope .mu. which is attributed to the total absorption coefficient of the tissue. Increments of .mu. due to deoxyhemoglobin absorption at 760 and 630 nm are used to calculate the concentration change. This permits the calculation of the path length for continuous light measurements of 2 cm for a particular geometry. Variation of the wavelength of the laser affords determination of a spectrum of changes in the tissue.