Effect of interleukin 1, macrophage colony-stimulating factor, and factor increasing monocytopoiesis on the production of leukocytes in mice.
Effect of interleukin 1, macrophage colony-stimulating factor, and factor increasing monocytopoiesis on the production of leukocytes in mice.
复制标题
白细胞介素 1、巨噬细胞集落刺激因子和单核细胞生成增加因子对小鼠白细胞生成的影响。
作者：
A. Annema;W. Sluiter;R. van Furth
本研究旨在确定增加单核细胞生成（FIM）因子是一种独特的因子，不同于白细胞介素1 （IL-1）和巨噬细胞集落刺激因子（M-CSF）在C3H/HeJ小鼠中对脂多糖无反应的粒细胞、淋巴细胞和单核细胞的产生。[3H]胸苷与所研究的细胞因子一起作为新形成细胞的标记物。根据每微升血液中白细胞的数量、白细胞的差异计数及其标记指数，通过放射自显像测定，计算循环中每一类标记白细胞的数量。为了比较各种细胞因子对白细胞产生的影响，我们计算了96小时内每种白细胞数量的曲线下面积（AUC）。结果表明，IL-1在2小时内引起循环粒细胞数量的增加，很可能是通过从骨髓储存池和血管边缘池中募集这些细胞。随之而来的是粒细胞的增加；单核细胞和淋巴细胞的产生不受IL-1的影响。注射M-CSF对体内粒细胞、淋巴细胞或单核细胞的产生没有显著影响。FIM特异性地刺激了单核细胞的产生，而对其他细胞系没有影响。由于FIM是由巨噬细胞在炎症部位吞噬后合成和分泌的，因此本研究表明，FIM是一种单因子，在急性需要更多单核细胞和（渗出）巨噬细胞时，作为一种远程调节因子向骨髓发出信号，以增加单核细胞的产生。
The present study was designed to establish that the factor increasing monocytopoiesis (FIM) is a unique factor that differs from interleukin 1 (IL-1) and macrophage colony-stimulating factor (M-CSF) in its effect on the production of granulocytes, lymphocytes, and monocytes in C3H/HeJ mice, which are unresponsive to lipopolysaccharide. [3H]thymidine, together with the cytokine under study, was used as a marker for newly formed cells. The number of each category of labeled leukocytes in the circulation was calculated from the number of leukocytes per microliter of blood, differential counts of the leukocytes, and their labeling indices, as determined by autoradiography. To compare the effect of the various cytokines on the production of leukocytes, the area under the curve (AUC) of the number of each category of leukocytes over a period of 96 h has been calculated. The results show that IL-1 causes, within 2 h, an increase in the number of circulating granulocytes, most probably by recruitment of these cells from the storage pool in the bone marrow and the marginating pool in the blood vessels. This is followed by an increased production of granulocytes; the production of monocytes and lymphocytes is not affected by IL-1. Administration of M-CSF had no significant effect on the production of granulocytes, lymphocytes, or monocytes in vivo. FIM specifically stimulated the production of monocytes and had no effect on the other cell lineages. Because FIM is synthesized and secreted by macrophages upon phagocytosis at the site of an inflammation, this study indicates that FIM is a monokine that acts as a long-range regulator to signal the bone marrow to increase monocyte production during an acute demand for more monocytes and (exudate) macrophages.