Osteoblast response to ovariectomy is enhanced in intrinsically high aerobic-capacity rats.
Osteoblast response to ovariectomy is enhanced in intrinsically high aerobic-capacity rats.
复制标题
在本质上高有氧能力的大鼠中，成骨细胞对卵巢切除的反应增强。
DOI：
10.1007/s00223-010-9457-x
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发表时间：
2011
影响因子：
4.2
通讯作者：
Kozloff,KM
中科院分区：
文献类型：
--
作者：
Goulet,GC;Halonen,NR;Koch,LG;Britton,SL;Zernicke,RF;Kozloff,KM
运动在促进骨骼健康中的作用通常归因于增加的机械负荷，其诱导功能适应。最近的证据表明，习惯性的有氧运动在细胞水平上也有影响。有氧能力对成骨细胞系细胞分化和功能以及骨骼表型的影响尚不清楚。使用大鼠模型的高容量和低容量的跑步者（HCRs和LCR，分别），其中一个内在的功能基因组差异有氧能力之间存在未经训练的动物，本研究评估的影响有氧能力的措施骨量和强度，以及成骨细胞活性卵巢切除术后。卵巢切除大鼠模仿雌激素耗竭的人类骨骼的临床特征，并代表了一个有价值的模型，用于研究成骨细胞活性的短期上调。我们假设，内在的高有氧能力将增加成骨细胞的反应，这将减轻激素戒断的有害影响。通过显微计算机断层扫描、力学测试和动态组织形态计量学评估股骨和胫骨。相对于LCR，HCR具有增强的股骨组织矿物质密度和估计的弹性模量。在卵巢切除术后4周，HCR表现出更强大的成骨细胞反应。骨形成标志物在HCR中的上调程度大于LCR，表明有氧能力在控制成骨细胞活性中的作用。这项研究和未来研究的结果将有助于确定细胞有氧代谢对骨骼健康的影响，这可能会导致针对骨骼疾病的新策略。
The role of exercise in promoting bone health is typically attributed to increased mechanical loading, which induces functional adaptation. Recent evidence suggests that habitual aerobic exercise has influence at the cellular level as well. The effect of aerobic capacity on osteoblast-lineage cell differentiation and function as well as skeletal phenotype is unknown. Using a rat model of high-capacity and low-capacity runners (HCRs and LCRs, respectively), in which an intrinsic functional genomic difference in aerobic capacity exists between nontrained animals, this study evaluated the effects of aerobic capacity on measures of bone mass and strength as well as osteoblast activity following ovariectomy. The ovariectomized rat emulates the clinical features of the estrogen-depleted human skeleton and represents a valuable model for studying short-term upregulation of osteoblast activity. We hypothesized that intrinsically high aerobic capacity would augment osteoblast response, which would mitigate the deleterious effects of hormone withdrawal. Femora and tibiae were assessed by micro-computed tomography, mechanical testing, and dynamic histomorphometry. HCRs had enhanced femoral tissue mineral density and estimated elastic modulus relative to LCRs. At 4 weeks postovariectomy, HCRs demonstrated a more robust osteoblast response. Markers of bone formation were upregulated to a greater extent in HCRs than LCRs, suggesting a role for aerobic capacity in governing osteoblast activity. Results from this and future studies will help to identify the influence of cellular aerobic metabolism on bone health, which may lead to new strategies for targeting diseases of the skeleton.