Improved radiochemical assay for carnitine and its derivatives in plasma and tissue extracts.
Improved radiochemical assay for carnitine and its derivatives in plasma and tissue extracts.
复制标题
改进了血浆和组织提取物中肉碱及其衍生物的放射化学测定。
作者：
J. Pace;R. Wannemacher;H. A. Neufeld
本文介绍了肝脏和肌肉高氯酸提取物中肉碱、短链和长链肉碱的测定方法。使用[1-14C]乙酰辅酶A，标记的乙酰肉碱的灵敏度为每50微升样品中L肉碱小于1~50nmol。在零下20摄氏度冷冻的样品在几个月内没有失去肉碱。肉碱(5nmol)加入50微升高氯酸提取液中的分析回收率为103+/-2%。这种方法可能适合于临床应用，如筛查肌肉和代谢紊乱患者。
We describe the measurement of carnitine and short-chain and long-chain acylcarnitines in perchloric acid extracts of liver and muscle. [1-14C]Acetyl-CoA is used, and labeled acetylcarnitine is measured with a sensitivity of less than 1 to 50 nmol of L-carnitine per 50-microliter sample. Samples frozen at -20 degrees C lost no carnitine during several months. Analytical recovery of carnitine (5 nmol) added to 50-microliter perchloric acid extracts of liver was 103 +/- 2%. This method may be suitable for clinical applications such as screening patients with muscle and metabolic disorders.