Pyoverdine and Pyochelin Measurements
Pyoverdine and Pyochelin Measurements
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DOI：
10.1007/978-1-4939-0473-0_24
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发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Schalk, Isabelle J.
中科院分区：
文献类型：
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作者：
Hoegy, Francoise;Mislin, Gaetan L. A.;Schalk, Isabelle J.
铁载体是一种小的有机螯合剂(分子量在200到2000 Da之间)，对铁有很高的亲和力(10(17)-10(43)M-1)。它们由细菌合成并分泌到环境中，以获得铁，铁是细菌生长的基本元素。铜绿假单胞菌在铁限制介质中产生的两种主要铁载体是吡喃佛丁(又称荧光素或假生物素)和绿球蛋白。提供了一种检测细菌培养物中吡喃甲苷和绿球蛋白含量的方法。这些方法都是基于这两个铁载体的光谱特性。
Siderophores are small organic chelators (of molecular weight between 200 and 2,000 Da), having a very high affinity for iron (10(17)-10(43) M-1). They are synthesized by bacteria and secreted into their environment in order to get access to iron, an essential element for bacterial growth. Pyoverdine (also called fluorescins or pseudobactins) and pyochelin are the two major siderophores produced by Pseudomonas aeruginosa in iron-limited media. Methods to specifically detect and measure the amount of pyoverdine and pyochelin in a bacterial culture are provided here. These methods are based on the spectral properties of these two siderophores.