2D and 3D collagen and fibrin biopolymers promote specific ECM and integrin gene expression by vascular smooth muscle cells
2D and 3D collagen and fibrin biopolymers promote specific ECM and integrin gene expression by vascular smooth muscle cells
复制标题
DOI：
10.1163/156856208786052380
复制
发表时间：
2008-01-01
影响因子：
3.6
通讯作者：
Stegemann, Jan P.
中科院分区：
文献类型：
--
作者：
Hong, Helen;Stegemann, Jan P.
I型胶原蛋白和纤维蛋白是再生医学领域中常用的聚合蛋白质，作为工程组织的基础基质。我们研究了血管平滑肌细胞（VSMC）的二维（2D）基板以及这些生物聚合物的三维（3D）矩阵的响应。研究了纯I型胶原、纯纤维蛋白和由胶原和纤维蛋白的1：1混合物组成的复合基质。使用定量RT-PCR在培养物中测定7天内三种ECM分子（I型和III型胶原和弹性蛋白原）和三种整联蛋白亚基（整联蛋白α 1、β 1和β 3）的相对基因表达。所有这些标志物基因的表达在3D矩阵中上调，相对于2D基板。原弹性蛋白、整合素α 1和整合素β 1在胶原基质中最高，而胶原III和整合素β 3在纯纤维蛋白中表达最高，胶原I在胶原-纤维蛋白复合材料中表达最高。这些特定ECM相关基因的组成和时间表达模式均提示伤口愈合反应。这些结果阐明了VSMC对2D和3D生物聚合物基质的短期反应，并与组织工程和心血管生物学相关。
Collagen Type I and fibrin are polymeric proteins commonly used in the field of regenerative medicine as the foundational matrix of engineered tissues. We examined the response of vascular smooth muscle cells (VSMC) to both two-dimensional (2D) substrates as well as three-dimensional (3D) matrices of these biopolymers. Pure collagen Type I, pure fibrin and composite matrices consisting of 1: 1 mixtures of collagen and fibrin were studied. Relative gene expression of three ECM molecules (collagen Type I and III, and tropoelastin) and three integrin subunits (integrins alpha 1, beta 1 and beta 3) was determined over 7 days in culture using quantitative RT-PCR. Expression of all of these marker genes was up-regulated in 3D matrices, relative to 2D substrates. Tropoelastin, integrin alpha 1 and integrin beta 1 were highest in collagen matrices, while collagen III and integrin beta 3 expression were highest in pure fibrin, and collagen I expression was highest in the collagen -fibrin composite materials. Both the compositional and temporal expression patterns of these specific ECM-related genes were suggestive of a wound healing response. These results illuminate the short-term responses of VSMC to 2D and 3D biopolymer matrices, and have relevance to tissue engineering and cardiovascular biology.