Effect of Platelet-rich Fibrin on Odontoblastic Differentiation in Human Dental Pulp Cells Exposed to Lipopolysaccharide
Effect of Platelet-rich Fibrin on Odontoblastic Differentiation in Human Dental Pulp Cells Exposed to Lipopolysaccharide
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DOI：
10.1016/j.joen.2016.11.002
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发表时间：
2017-03-01
影响因子：
4.2
通讯作者：
Jung, Ji-Yeon
中科院分区：
文献类型：
--
作者：
Kim, Jae-Hwan;Woo, Su-Mi;Jung, Ji-Yeon
前言：富血小板纤维蛋白(PRF)是一种自体纤维蛋白基质，含有血小板、白细胞和生长因子，可控制炎症和促进愈合过程。本研究旨在探讨PRF对内毒素诱导的人牙髓细胞向成牙本质细胞分化的影响。方法：采用逆转录聚合酶链式反应和免疫印迹分析方法检测内毒素和PRF提取物(PRFe)作用前后HDPC上炎性细胞因子和黏附分子的基因表达。碱性磷酸酶染色检测成牙本质细胞的碱性磷酸酶活性，茜素红S染色检测成牙本质细胞分化相关基因的表达及矿化程度。结果：PRFe可显著降低脂多糖刺激的HDPC中IL-1β、IL-6和IL-8的表达。此外，PrFe还可抑制脂多糖诱导的HDPC血管细胞黏附分子1的上调和细胞内黏附分子1的产生。PRFe可促进牙本质涎磷蛋白和牙本质基质酸性磷酸蛋白1的表达，增强碱性磷酸酶活性和矿化能力。结论：PRF不仅能抑制脂多糖诱导的HDPC的炎症反应，而且能促进成牙本质细胞的分化。
Introduction: Platelet-rich fibrin (PRF), as an autologous fibrin matrix, is known to contain platelets, leukocytes, and growth factors to control inflammation and to facilitate the healing process. The purpose of this study was to investigate the effects of PRF on odontoblastic differentiation in human dental pulp cells (HDPCs) treated with lipopolysaccharide (LPS). Methods: Gene expression of inflammatory cytokines and adhesion molecules on the HDPCs cultured with or without LPS and PRF extract (PRFe) were evaluated by reverse transcription polymerase chain reaction and Western blot analysis. In addition, odontoblastic differentiation was determined by measuring alkaline phosphatase (ALP) activity using ALP staining, the expression of odontogenesis-related genes, and the extent of mineralization using alizarin red S staining. Results: Treatment with PRFe significantly attenuated the LPS-stimulated expression of interleukin (IL)-1 beta, IL-6, and IL-8 in HDPCs. In addition, PRFe inhibited the up-regulation of vascular cell adhesion molecule 1 and the production of intracellular adhesion molecule 1 in HDPCs exposed to LPS. Expression of dentin sialophosphoprotein and dentin matrix acidic phosphoprotein 1, ALP activity, and mineralization were enhanced by PRFe in LPS-treated HDPCs. Conclusions: These results suggest that PRF has effects associated not only with inhibition of inflammation in HDPCs exposed to LPS but also stimulation of odontoblastic differentiation.