Layer-by-Layer Self-Assembling Gold Nanorods and Glucose Oxidase onto Carbon Nanotubes Functionalized Sol-Gel Matrix for an Amperometric Glucose Biosensor.
Layer-by-Layer Self-Assembling Gold Nanorods and Glucose Oxidase onto Carbon Nanotubes Functionalized Sol-Gel Matrix for an Amperometric Glucose Biosensor.
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用于安培葡萄糖生物传感器的碳纳米管功能化溶胶-凝胶基质上的逐层自组装金纳米棒和葡萄糖氧化酶
DOI：
10.3390/nano5031544
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发表时间：
2015-09-18
期刊：
影响因子：
--
通讯作者：
Ji Y
中科院分区：
文献类型：
--
作者：
Wu B;Hou S;Miao Z;Zhang C;Ji Y
通过将金纳米棒（AuNR）和葡萄糖氧化酶（GOD）逐层自组装到单壁碳纳米管（SWCNT）功能化的三维溶胶-凝胶基质上，制备了一种新型电流型葡萄糖生物传感器。首先将含有 SWCNTs 的硫醇化水性硅溶胶组装在清洁的 Au 电极表面，然后重复 AuNRs 和 GOD 的交替自组装，将 AuNRs-GOD 多层膜组装到 SWCNTs 功能化的硅胶上，以优化生物传感器。在所得的葡萄糖生物传感器中，四层 AuNRs-GOD 修饰电极表现出最佳性能。 sol-SWCNTs-(AuNRs-GOD)4/Au生物传感器表现出0.01-8 mM葡萄糖的良好线性范围、1.08 μA/mM的高灵敏度以及4秒内的快速电流响应。所提出的葡萄糖生物传感器的良好性能主要归因于三维溶胶-凝胶基质和立体自组装膜的优点以及一维纳米结构SWCNT和AuNR的自然特征。这项研究可能为制造高性能酶基生物传感器提供一种新的简便方法。
A novel amperometric glucose biosensor was fabricated by layer-by-layer self-assembly of gold nanorods (AuNRs) and glucose oxidase (GOD) onto single-walled carbon nanotubes (SWCNTs)-functionalized three-dimensional sol-gel matrix. A thiolated aqueous silica sol containing SWCNTs was first assembled on the surface of a cleaned Au electrode, and then the alternate self-assembly of AuNRs and GOD were repeated to assemble multilayer films of AuNRs-GOD onto SWCNTs-functionalized silica gel for optimizing the biosensor. Among the resulting glucose biosensors, the four layers of AuNRs-GOD-modified electrode showed the best performance. The sol-SWCNTs-(AuNRs-GOD)4/Au biosensor exhibited a good linear range of 0.01–8 mM glucose, high sensitivity of 1.08 μA/mM, and fast amperometric response within 4 s. The good performance of the proposed glucose biosensor could be mainly attributed to the advantages of the three-dimensional sol-gel matrix and stereo self-assembly films, and the natural features of one-dimensional nanostructure SWCNTs and AuNRs. This study may provide a new facile way to fabricate the enzyme-based biosensor with high performance.