Visualization of the Linear and Spatial Organization of Chromosomes in Mosquitoes.
Visualization of the Linear and Spatial Organization of Chromosomes in Mosquitoes.
复制标题
蚊子染色体线性和空间组织的可视化。
DOI：
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复制
发表时间：
2022
影响因子：
--
通讯作者：
M. Sharakhova
中科院分区：
文献类型：
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作者：
Jiangtao Liang;Simon M Bondarenko;I. Sharakhov;M. Sharakhova
蚊子是疟疾、登革热、寨卡病毒、西尼罗热和淋巴丝虫病等危险人类疾病的传播媒介。可视化蚊子染色体的线性和空间组织对于理解基因组的结构和功能具有重要意义。利用染色体倒位作为群体遗传学研究的标记，可以深入了解蚊子的适应和进化。细胞遗传学方法有助于染色体尺度基因组组装的发展，这是研究蚊子生物学和设计新的媒介控制策略的有用工具。荧光原位杂交是一种定位染色体线性结构和细胞核空间组织内特定DNA序列的强大技术。在这里，我们介绍了在我们的实验室中使用的染色体可视化方案及其在蚊子中的应用。
Mosquitoes are vectors of dangerous human diseases such as malaria, dengue, Zika, West Nile fever, and lymphatic filariasis. Visualization of the linear and spatial organization of mosquito chromosomes is important for understanding genome structure and function. Utilization of chromosomal inversions as markers for population genetics studies yields insights into mosquito adaptation and evolution. Cytogenetic approaches assist with the development of chromosome-scale genome assemblies that are useful tools for studying mosquito biology and for designing novel vector control strategies. Fluorescence in situ hybridization is a powerful technique for localizing specific DNA sequences within the linear chromosome structure and within the spatial organization of the cell nucleus. Here, we introduce protocols used in our laboratories for chromosome visualization and their application in mosquitoes.
DOI：
10.1080/19491034.2020.1819704
发表时间：
2020-12
期刊：
Nucleus (Austin, Tex.)
影响因子：
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作者：
Bondarenko SM;Sharakhov IV
通讯作者：
Sharakhov IV
影响因子：
4.8
作者：
Belton, Jon-Matthew;McCord, Rachel Patton;Gibcus, Johan Harmen;Naumova, Natalia;Zhan, Ye;Dekker, Job
通讯作者：
Dekker, Job
影响因子：
6
作者：
George, Phillip;Kinney, Nicholas A.;Sharakhov, Igor V.
通讯作者：
Sharakhov, Igor V.