CHARACTERIZATION OF 4 NEW ADENOVIRUS SEROTYPES ISOLATED FROM CHIMPANZEE TISSUE EXPLANTS
CHARACTERIZATION OF 4 NEW ADENOVIRUS SEROTYPES ISOLATED FROM CHIMPANZEE TISSUE EXPLANTS
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DOI：
10.1093/oxfordjournals.aje.a121308
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发表时间：
1971-01-01
影响因子：
5
通讯作者：
GAJDUSEK, DC
中科院分区：
文献类型：
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作者：
BASNIGHT, M;ROGERS, NG;GAJDUSEK, DC
巴斯奈特，M.，Jr.(NINDS，NIH，马里兰州贝塞斯达20014)，N.G.罗杰斯，C.J.吉布斯，Jr.和D.C.Gajdusek。从黑猩猩组织外植体中分离的四种新的腺病毒血清型的特征.美国期刊94：166-171,1971.-从实验性库鲁病和克雅病晚期的黑猩猩的移植淋巴结中分离到四种新的潜伏性腺病毒血清型.这些病毒在人胚胎肾细胞培养中诱导典型的腺病毒细胞病理学改变，并在人腺病毒抗血清存在下固定补体。它们具有腺病毒的生化和物理特性；它们在人类细胞培养物中比在猿源细胞培养物中繁殖得更好。它们不能被已知的人和猿腺病毒类型的抗血清中和，并且在免疫学上彼此不同。这些病毒被认为不是从黑猩猩身上分离出来的实验性库鲁病或克雅病的病原体，尽管这些动物在疾病过程中产生了同源的中和抗体。在正常黑猩猩中也发现了针对这些新的腺病毒血清型的中和抗体，但在与黑猩猩密切接触1-3年的动物饲养员的血清中未发现中和抗体。
Basnight, M., Jr. (NINDS, NIH, Bethesda, Md. 20014), N. G. Rogers, C. J. Gibbs, Jr. and D. C. Gajdusek. haracterization of four new adenovirus serotypes isolated from chimpanzee tissue explants.Amer J Epidem94: 166–171, 1971.—Four new latent adenovirus serotypes were isolated from explanted lymph nodes of chimpanzees in the late stages of experimental kuru and Creutzfeldt-Jakob disease. These viruses induced typical adenovirus cytopathology in human embryonic kidney cell cultures and fixed complement in the presence of human adenovirus antiserum. They had the biochemical and physical characteristics of adenoviruses; they propagated better in cell cultures of human than in those of simian origin. They were not neutralized by antisera of known human and simian adenovirus types and were immunologically distinct from each other. The viruses are not believed to be the agents of the experimental kuru or Creutzfeldt-Jakob disease in the chimpanzees from which they were isolated, although these animals developed homologous neutralizing antibodies during the course of their disease. Neutralizing antibodies to these new adenovirus serotypes were also found in normal chimpanzees, but were absent from sera of animal handlers who had been in close contact with the chimpanzees for 1–3 years.