T type 1/type 2 subsets balance in B-cell chronic lymphocytic leukemia - the three-color flow cytometry analysis
T type 1/type 2 subsets balance in B-cell chronic lymphocytic leukemia - the three-color flow cytometry analysis
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DOI：
10.1016/s0145-2126(01)00194-1
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发表时间：
2002-07-01
影响因子：
2.7
通讯作者：
Wasik, E
中科院分区：
文献类型：
--
作者：
Podhorecka, M;Dmoszynska, A;Wasik, E
免疫细胞功能的损害以及细胞因子网络的改变是B细胞慢性淋巴细胞白血病（B-CLL）恶性细胞积聚和继发性免疫缺陷的原因。为了拓宽对B-CLL患者免疫系统的认识，我们试图评估B-CLL患者与健康个体相比的Th 1/Th 2和Tc 1/Tc 2平衡以及疾病进展过程中这种模式的变化。采用三色流式细胞术检测外周血CD 3 + CD 4+和CD 3 + CD 8+细胞中IL-4和IFN-γ的表达，结果表明B-CLL患者以T 1型细胞和T细胞介导的免疫为主，但随着疾病进展，T细胞介导的免疫向T 2型转移。(C)2002爱思唯尔科技有限公司版权所有。
The impairments in immune cell functions as well as changes in cytokine network are the Cause of malignant cell accumulation and secondary immune deficiencies in B-cell chronic lymphocytic leukemia (B-CLL). To broaden the knowledge of immune system ill B-CLL we tried to evaluate the Th1/Th2 and Tc1/Tc2 balance in B-CLL-patients in comparison with healthy individuals and changes of this pattern during disease progression. The three-color flow cytometry technique was used to analyze IL-4 and IFNgamma expression in peripheral blood CD3+CD4+ and CD3+CD8+ cells.The results indicate the dominance of T type 1 cells and T-cell-mediated immunity in B-CLL patients that is however shifted towards T type 2 during disease progression. (C) 2002 Elsevier Science Ltd. All rights reserved.