Identification of an intracellular pyrimidine-specific endoribonuclease from Bacillus subtilis.
Identification of an intracellular pyrimidine-specific endoribonuclease from Bacillus subtilis.
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枯草芽孢杆菌胞内嘧啶特异性内切核糖核酸酶的鉴定。
DOI：
10.1128/jb.175.20.6717-6720.1993
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发表时间：
1993
影响因子：
3.2
通讯作者：
Kennell,D
中科院分区：
文献类型：
--
作者：
Mathur,S;Cannistraro,VJ;Kennell,D
从枯草芽孢杆菌中分离出两种胞内核糖核酸酶，这两种核糖核酸酶可以通过强阴、阳离子交换树脂分离得到。一个可以裂解任何磷酸二酯键，而第二个只能裂解嘧啶-N键。该酶具有约15 kDa的N-嘧啶专一性，最适pH约为6.2，降解C-C键的速度约为U-U键的10倍，对单链DNA完全失活。该酶被称为RNaseC，可能是第一个报道的枯草杆菌广特异性内切核酸酶。
Two intracellular RNases which were easily separated by fractionation on strong anion- or cation-exchange resins were identified from Bacillus subtilis. One cleaved any phosphodiester bond, while the second cleaved only pyrimidine-N bonds. The enzyme with pyrimidine-N specificity was approximately 15 kDa, had a pH optimum of approximately 6.2, degraded C-C bonds approximately 10 times faster than U-U bonds, and was completely inactive against single-stranded DNA. The enzyme is called RNase C and may be the first reported broad-specificity endoribonuclease from B. subtilis.