Clinical and Biochemical Evaluation of Lozenges Containing Lactobacillus reuteri as an Adjunct to Non-Surgical Periodontal Therapy in Chronic Periodontitis
Clinical and Biochemical Evaluation of Lozenges Containing Lactobacillus reuteri as an Adjunct to Non-Surgical Periodontal Therapy in Chronic Periodontitis
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DOI：
10.1902/jop.2015.140612
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发表时间：
2015-06-01
影响因子：
4.3
通讯作者：
Yilmaz, Selcuk
中科院分区：
文献类型：
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作者：
Ince, Gizem;Gursoy, Hare;Yilmaz, Selcuk
背景：本研究评估了含罗伊氏乳杆菌的益生菌补充剂辅助慢性牙周炎 (CP) 患者初始牙周治疗的临床和生化参数的影响。方法：纳入 30 名 CP 患者并分为两组。每个患者的每个象限都有≥2颗牙齿，每颗牙齿的邻位点探诊深度（PD）为5至7毫米，牙龈指数（GI）≥2。测试组接受洗牙和根面平整（SRP）和含益生菌含片。对照组接受 SRP 和安慰剂含片。测量斑块指数 (PI)、GI、探诊出血 (BOP)、PD 和附着增益。采集龈沟液（GCF）样本，通过酶联免疫吸附测定分析基质金属蛋白酶（MMP）-8和金属蛋白酶组织抑制剂（TIMP）-1。所有评估均在基线以及第 21 天、第 90 天、第 180 天和第 360 天进行。结果：PI、GI、BOP 和 PD 的组间比较差异在所有时间点均显着（P < 0.05），有利于测试组。直至第 180 天，GCF MMP-8 水平降低和 TIMP-1 水平升高均显着（P < 0.05）。在第 90 天、第 180 天和第 360 天，测试组的依恋增益平均值显着高于对照组。 结论：含有罗伊氏乳杆菌的含片可能是中度深度脑瘫患者的有用补充剂。低 MMP-8 和高 TIMP-1 水平可能表明含片在减少炎症相关标志物方面的作用，直至第 180 天。
Background: This study evaluates the effects on clinical and biochemical parameters of Lactobacillus reuteri-containing probiotic supplementation adjunctive to initial periodontal therapy in patients with chronic periodontitis (CP).Methods: Thirty patients with CP were included and divided into two groups. Every patient had, in each quadrant, >= 2 teeth each with approximal sites with a probing depth (PD) of 5 to 7 mm and gingival index (GI) of >= 2. The test group received scaling and root planing (SRP) and probiotic-containing lozenges. The control group received SRP and placebo lozenges. Plaque index (PI), GI, bleeding on probing (BOP), PD, and attachment gain were measured. Gingival crevicular fluid (GCF) was sampled for the analysis of matrix metalloproteinase (MMP)-8 and tissue inhibitor of metalloproteinase (TIMP)-1 by enzyme-linked immunosorbent assay. All evaluations were performed at baseline and on days 21, 90, 180, and 360.Results: Differences in intergroup comparisons of PI, GI, BOP, and PD were found to be significant (P < 0.05) in favor of the test group at all time points. Decreased GCF MMP-8 levels and increased TIMP-1 levels were found to be significant up to day 180 (P < 0.05). Mean values of attachment gain were significantly higher in the test group compared with the control group on days 90, 180, and 360.Conclusions: Lozenges containing L. reuteri may be a useful supplement in moderately deep pockets of patients with CP. Low MMP-8 and high TIMP-1 levels may indicate the role of the lozenges in reduction of inflammation-associated markers up to day 180.