Molecular crowding improves bead-based padlock rolling circle amplification
Molecular crowding improves bead-based padlock rolling circle amplification
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DOI：
10.1016/j.ab.2016.12.002
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发表时间：
2017-02-15
影响因子：
2.9
通讯作者：
Sato, Kae
中科院分区：
文献类型：
--
作者：
Sasaki, Naoki;Gunji, Yoshitaka;Sato, Kae
在聚乙二醇（PEG）形成的分子拥挤环境中，采用基于小球的挂锁滚环扩增技术（RCA）进行了超灵敏、高精度的DNA检测。生成的RCA产物数量增加，并呈现钟形依赖于PEG浓度。磁珠实验表明，DNA连接和杂交的便利化是观察到的增加的主要原因。该技术的选择性在PEG存在下保持不变。该技术操作简单，可用于医学诊断和食品分析等多种领域的目标DNA检测，具有较高的准确性和灵敏度。(C) 2016 Elsevier Inc.版权所有。
Bead-based padlock rolling circle amplification (RCA), an ultrasensitive and accurate DNA detection technique, was conducted in a molecular crowding environment created by poly(ethylene glycol) (PEG). The number of RCA products generated increased and exhibited a bell-shaped dependence on PEG concentration. Experiments using magnetic beads suggested that facilitation of DNA ligation and hybridization is the main reason for the observed increase. Selectivity of the technique was retained in the presence of PEG. This technique is simple and can be utilized to detect target DNA with high accuracy and sensitivity in a variety of areas such as medical diagnosis and food analysis. (C) 2016 Elsevier Inc. All rights reserved.