Characterization of thymic stromal-derived lymphopoietin (TSLP) in murine B cell development in vitro
Characterization of thymic stromal-derived lymphopoietin (TSLP) in murine B cell development in vitro
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DOI：
10.1002/eji.1830260103
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发表时间：
1996-01-01
影响因子：
5.4
通讯作者：
Paige, CJ
中科院分区：
文献类型：
--
作者：
Ray, RJ;Furlonger, C;Paige, CJ
B细胞的发育既依赖于与基质细胞的直接相互作用，也依赖于它们所分泌的细胞因子。这些相互作用调节B细胞分化的确切机制目前尚不清楚。我们在此报道一种新型生长因子——胸腺基质衍生的淋巴细胞生成素（TSLP）在体外支持B细胞发育方面能够替代白细胞介素 - 7（IL - 7）的活性。研究发现TSLP可促进来自15天胎肝的定向B220⁻ B细胞祖细胞的增殖和分化。对这些细胞的表型分析表明，它们处于前B细胞分化阶段，并表达在IL - 7中培养的前B细胞所特有的细胞表面标志物。TSLP在支持B淋巴细胞从未定向的双潜能前体的发育进程中能够替代IL - 7的活性。在缺乏TSLP或IL - 7的情况下，产生成熟B淋巴细胞的细胞后代无法从这些双潜能前体发育而来。此外，TSLP在支持CBA/N小鼠的B细胞祖细胞所表现出的持续增殖反应方面能够替代IL - 7。这些结果共同表明，在体外B细胞发育过程中，TSLP能够替代对IL - 7的需求。
B cell development is dependent on both direct interactions with stromal cells and their secreted cytokines. The precise mechanisms by which these interactions regulate B cell differentiation are currently unknown. We report here that a novel growth factor thymic stromal-derived lymphopoietin (TSLP) can replace the activity of interleukin-7 (IL-7) in supporting B cell development in vitro. TSLP was found to promote the proliferation and differentiation of committed B220(-) B cell progenitors from day 15 fetal liver. Phenotypic analysis of these cells revealed that they are at the pro-B cell stage of differentiation and express cell surface markers characteristic of pro-B cells cultured in IL-7. TSLP can replace the activity of IL-7 in supporting the progression of B lymphocytes from uncommitted bipotential precursors. In the absence of either TSLP or IL-7, the progeny of cells that give rise to mature B lymphocytes fail to develop from these bipotential precursors. Moreover, TSLP can substitute for IL-7 in supporting the sustained proliferative response exhibited by B cell progenitors from CBA/N mice. Together these results show that TSLP can replace the requirement for IL-7 during in vitro B cell development.