A Comprehensive Analysis of miRNA/isomiR Expression with Gender Difference.
A Comprehensive Analysis of miRNA/isomiR Expression with Gender Difference.
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miRNA/isomiR 表达与性别差异的综合分析
DOI：
10.1371/journal.pone.0154955
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zou Q
中科院分区：
文献类型：
--
作者：
Guo L;Liang T;Yu J;Zou Q
尽管microRNA（miRNAs）作为表观遗传调控分子已被广泛研究，但很少有研究关注miRNAs和isomiR表达水平的性别差异。在这项研究中，我们的目标是通过对基于不同人类疾病和组织的小RNA测序数据集的全面分析，了解性别差异与miRNA/isomiR表达之间的潜在关系。基于来自男性和女性的特定样本，我们确定了一些miRNA可能在不同组织和性别之间差异表达。因此，这些miRNAs可能在男性和女性之间表现出不一致甚至相反的表达。根据去调控的miRNA表达谱，选择一些显性表达的miRNA位点，使用显性isomiR的比率分析isomiR表达模式。在一些miRNA位点中，isomiR在肿瘤和正常样品之间以及男性和女性样品之间显示出统计学显著性，这表明isomiR表达模式并不总是不变的，但可能在男性和女性之间以及在不同组织、肿瘤和正常样品之间变化。这种差异暗示了miRNA表达的波动及其在isomiR水平上的详细表达。这种差异也表明性别差异可能是影响疾病相关miRNAs和isomiRs筛选的重要因素。这项研究表明，miRNA/isomiR表达和性别差异可能比以前假设的更复杂，应根据男性或女性的特定样本进一步研究。
Although microRNAs (miRNAs) have been widely studied as epigenetic regulation molecules, fewer studies focus on the gender difference at the miRNA and isomiR expression levels. In this study, we aim to understand the potential relationships between gender difference and miRNA/isomiR expression through a comprehensive analysis of small RNA-sequencing datasets based on different human diseases and tissues. Based on specific samples from males and females, we determined that some miRNAs may be diversely expressed between different tissues and genders. Thus, these miRNAs may exhibit inconsistent and even opposite expression between males and females. According to deregulated miRNA expression profiles, some dominantly expressed miRNA loci were selected to analyze isomiR expression patterns using rates of dominant isomiRs. In some miRNA loci, isomiRs showed statistical significance between tumor and normal samples and between males and females samples, suggesting that isomiR expression patterns are not always invariable but may vary between males and females, as well as among different tissues, tumors, and normal samples. The divergence implicates the fluctuation in the expression of miRNA and its detailed expression at the isomiR levels. The divergence also indicates that gender difference may be an important factor that affects the screening of disease-associated miRNAs and isomiRs. This study suggests that miRNA/isomiR expression and gender difference may be more complex than previously assumed and should be further studied according to specific samples from males or females.