Establishment and characterization of a novel canine B-cell line derived from a spontaneously occurring diffuse large cell lymphoma
Establishment and characterization of a novel canine B-cell line derived from a spontaneously occurring diffuse large cell lymphoma
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DOI：
10.1016/j.leukres.2010.01.021
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发表时间：
2010-07-01
影响因子：
2.7
通讯作者：
Saalmueller, Armin
中科院分区：
文献类型：
--
作者：
Ruetgen, Barbara C.;Hammer, Sabine E.;Saalmueller, Armin
自发肿瘤细胞系是研究肿瘤细胞生物学和开发新型抗癌药物的重要工具。犬淋巴瘤细胞系的分离和繁殖是困难的，因此只有少数可用。现在我们已经建立了一个新的b细胞淋巴瘤细胞系CLBL-1从犬确诊的IV期弥漫性大细胞淋巴瘤。CLBL-1细胞免疫表型显示CD11a、CD79 α cy、CD45、CD45RA、MHCⅱ阳性，CD3、CD4、CD5、CD8、CD11d、CD14、CD21、CD34、CD56和t细胞受体- γ δ (tcr - γ δ)阴性。对tcr - γ和免疫球蛋白重链（IgH）基因重排的PCR分析得出了IgH基因的单克隆结果。此外，通过对16个阳性细菌克隆的测序，证实了IgH基因重排的克隆性。由于犬淋巴瘤在许多方面与人类的非霍奇金淋巴瘤（NHL）相似，这种新的细胞系将促进人类和狗淋巴瘤的转化和比较研究。(C) 2010 Elsevier Ltd.版权所有。
Cell lines derived from spontaneous tumors serve as a research tool for cancer cell biology and new anti-cancer drug development. Isolation and propagation of canine lymphoma cell lines is difficult, thus only a few are available. Now we have established a new B-cell lymphoma cell line CLBL-1 from a dog with confirmed stage IV diffuse large cell lymphoma. Immunophenotyping of these CLBL-1 cells showed positive staining for CD11a, CD79 alpha cy, CD45, CD45RA, MHC II and cells were negative for CD3, CD4, CD5, CD8, CD11d, CD14, CD21, CD34, CD56 and T-cell receptor-gamma delta (TCR-gamma delta). PCR analysis for TCR-gamma and immunoglobulin heavy chain (IgH) gene rearrangements yielded a monoclonal result for the IgH gene. Furthermore, the clonality of IgH gene rearrangement was confirmed by sequencing of 16 positive bacterial clones. As canine lymphoma resembles non-Hodgkin's lymphoma (NHL) in humans in many respects, this new cell line, will promote translational and comparative lymphoma research in humans and dogs. (C) 2010 Elsevier Ltd. All rights reserved.