Tetramethylpyrazine Protects Retinal Capillary Endothelial Cells (TR-iBRB2) against IL-1β-Induced Nitrative/Oxidative Stress.
Tetramethylpyrazine Protects Retinal Capillary Endothelial Cells (TR-iBRB2) against IL-1β-Induced Nitrative/Oxidative Stress.
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DOI：
10.3390/ijms160921775
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发表时间：
2015-09-09
影响因子：
5.6
通讯作者：
Zhu L
中科院分区：
文献类型：
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作者：
Zhu X;Wang K;Zhang K;Tan X;Wu Z;Sun S;Zhou F;Zhu L
血-视网膜屏障（BRB）的破坏是糖尿病视网膜病变（DR）的主要原因之一。在DR的发病过程中，促炎因子白细胞介素1β（IL-1β）参与了BRB的破坏。本研究观察了中药川芎的主要活性成分川芎嗪（TMP）对IL-1β诱导的大鼠视网膜毛细血管内皮细胞TR-iBRB 2细胞死亡的保护作用。我们的研究结果表明，IL-1β通过诱导硝化/氧化应激诱导TR-iBRB 2细胞功能障碍;然而，TMP预处理减弱了这种作用。TMP的细胞保护作用可能是通过抑制诱导型一氧化氮合酶（iNOS）表达和白细胞停滞以及抑制活性氧（ROS）产生、线粒体功能障碍和MAPKs活化来介导的。这些发现有助于更好地理解TMP在DR中的保护作用，并为将来TMP治疗此类疾病的治疗开发奠定了基础。
Blood-retinal barrier (BRB) breakdown is one of the primary causes of diabetic retinopathy (DR). The pro-inflammatory factor interleukin-1β (IL-1β) was reported to be involved in the induction of BRB breakdown during the pathogenesis of DR. In the present study, we investigated the protective effect of tetramethylpyrazine (TMP), a major active component of the traditional herb Ligusticum chuanxiong, on IL-1β-induced cell death of the rat retinal capillary endothelial TR-iBRB2 cells. Our results showed that IL-1β-induced cell dysfunction in TR-iBRB2 cells via inducing nitrative/oxidative stress; however, such effect was attenuated with the pre-treatment of TMP. The cellular protective effect of TMP was likely to be mediated through the inhibition of inducible nitric oxide synthase (iNOS) expression and leukostasis as well as suppression of reactive oxygen species (ROS) generation, mitochondrial dysfunction and MAPKs activation. These findings significantly contribute to a better understanding of the protective effect of TMP in DR and form the basis of the therapeutic development of TMP in treating such disease in the future.