Heterogeneity and seroprevalence of a newly identified avian hepatitis E virus from chickens in the United States
Heterogeneity and seroprevalence of a newly identified avian hepatitis E virus from chickens in the United States
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DOI：
10.1128/jcm.40.11.4197-4202.2002
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发表时间：
2002-11-01
影响因子：
9.4
通讯作者：
Meng, XJ
中科院分区：
文献类型：
--
作者：
Huang, FF;Haqshenas, G;Meng, XJ
我们最近在美国从患有肝炎-脾肿大综合征（HS综合征）的鸡中鉴定并鉴定了一种新病毒，命名为禽戊型肝炎病毒（禽HEV）。禽戊型肝炎病毒与人类和猪的戊型肝炎病毒在遗传上相关，但不同。为了确定鸡群中禽戊型肝炎病毒感染的遗传变异程度和血清阳性率，我们从HS综合征鸡群中遗传鉴定并鉴定了另外11株禽戊型肝炎病毒分离株，并评估了来自5个州（加州、科罗拉多、康涅狄格、弗吉尼亚和威斯康星州）76个不同鸡群的1,276只不同年龄和品种鸡的禽戊型肝炎病毒抗体的流行率。利用截短的重组禽戊型肝炎病毒ORF 2抗原建立了酶联免疫吸附试验，并用于测定禽戊型肝炎病毒血清阳性率。约71%的鸡群和30%的鸡在研究中检测到禽戊型肝炎病毒抗体阳性。约17%的小于18周龄的鸡是血清阳性，而约36%的成年鸡是血清阳性。通过使用逆转录-PCR（RT-PCR）检测，我们测试了21个胆汁样本，从鸡HS综合征在加州，康涅狄格州，纽约，和威斯康星州的存在下，禽戊型肝炎病毒RNA。在21份胆汁样品中，12份经电子显微镜（EM）检测为30- 35 nm HEV样病毒颗粒阳性。12份EM阳性胆汁标本中有11份RT-PCR阳性，9份EM阴性胆汁标本中有6份RT-PCR阳性。测定了11株禽戊型肝炎病毒（HEV）解旋酶基因372 bp的序列。序列分析表明，11株禽戊型肝炎病毒的核苷酸序列同源性为78 ~ 100%，与禽戊型肝炎病毒原型株的同源性为79 ~ 88%，与鸡大肝脾病病毒的同源性为76 ~ 80%，与人和猪戊型肝炎病毒的同源性为56 ~ 61%。这项研究的数据表明，像猪和人的戊型肝炎病毒，禽戊型肝炎病毒分离株是遗传异质性和禽戊型肝炎病毒感染是在美国鸡群的地方流行病。
We recently identified and characterized a novel virus, designated avian hepatitis E virus (avian HEV), from chickens with hepatitis-splenomegaly syndrome (HS syndrome) in the United States. Avian HEV is genetically related to but distinct from human and swine HEVs. To determine the extent of genetic variation and the seroprevalence of avian HEV infection in chicken flocks, we genetically identified and characterized 11 additional avian HEV isolates from chickens with HS syndrome and assessed the prevalence of avian HEV antibodies from a total of 1,276 chickens of different ages and breeds from 76 different flocks in five states (California, Colorado, Connecticut, Virginia, and Wisconsin). An enzyme-linked immunosorbent assay using a truncated recombinant avian HEV ORF2 antigen was developed and used to determine avian HEV seroprevalence. About 71% of chicken flocks and 30% of chickens tested in the study were positive for antibodies to avian HEV. About 17% of chickens younger than 18 weeks were seropositive, whereas about 36% of adult chickens were seropositive. By using a reverse transcription-PCR (RT-PCR) assay, we tested 21 bile samples from chickens with HS syndrome in California, Connecticut, New York, and Wisconsin for the presence of avian HEV RNA. Of the 21 bile samples, 12 were positive for 30- to 35-nm HEV-like virus particles by electron microscopy (EM). A total of 11 of the 12 EM-positive bile samples and 6 of the 9 EM-negative bile samples were positive for avian HEV RNA by RT-PCR. The sequences of a 372-bp region within the helicase gene of 11 avian HEV isolates were determined. Sequence analyses revealed that the 11 field isolates of avian HEV had 78 to 100% nucleotide sequence identities to each other, 79 to 88% identities to the prototype avian HEV, 76 to 80% identities to chicken big liver and spleen disease virus, and 56 to 61% identities to other known strains of human and swine HEV. The data from this study indicated that, like swine and human HEVs, avian HEV isolates are genetically heterogenic and that avian HEV infection is enzoonotic in chicken flocks in the United States.