Usefulness of spoligotyping to discriminate IS6110 low-copy-number Mycobacterium tuberculosis complex strains cultured in Denmark
Usefulness of spoligotyping to discriminate IS6110 low-copy-number Mycobacterium tuberculosis complex strains cultured in Denmark
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DOI：
10.1128/jcm.37.8.2602-2606.1999
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发表时间：
1999-08-01
影响因子：
9.4
通讯作者：
Miörner, H
中科院分区：
文献类型：
--
作者：
Bauer, J;Andersen, ÅB;Miörner, H
从1992年起，在常规基础上通过IS 6110限制性片段长度多态性（RFLP）分析了在丹麦培养的结核分枝杆菌复合体菌株。由于结核病移民的涌入，仅携带1 - 5个IS 6110拷贝的菌株数量稳步增加。由于IS 6110指纹图谱对这些菌株的区分能力很差，我们采用最近描述的基于PCR的称为spoligotyping的方法对所有低拷贝数菌株进行了额外的基因分型。总共对311株临床菌株进行了分型：14株牛分枝杆菌BCG，48株M。bovis和249 M. Spoligotyping能正确区分牛支原体和牛支原体BCG与结核分枝杆菌，但不能区分牛支原体。牛支原体、牛卡介苗、全支原体。牛BCG菌株具有相同的spoligotype模式。低拷贝数M.结核分枝杆菌菌株的RFLP分型率高于IS 6110指纹分型率。两种技术结合后，聚类率下降到55%。这两种技术的结合可能对研究IS 6110基因拷贝数较少的结核分枝杆菌菌株的流行病学有价值。
Mycobacterium tuberculosis complex strains cultured in Denmark have been analyzed by IS6110 restriction fragment length polymorphism (RFLP) on a routine basis from 1992 and onwards, Due to the influx of immigrants with tuberculosis, the number of strains harboring only one to five copies of IS6110 has increased steadily. Since the discriminatory power of IS6110 fingerprinting for such strains is poor, we have performed additional genotyping of all low-copy-number strains by the recently described PCR-based method known as spoligotyping, A total of 311 clinical strains were typed: 14 Mycobacterium bovis BCG, 48 M. bovis, and 249 M. tuberculosis strains, Spoligotyping correctly differentiated M, bovis and M, bovis BCG from M, tuberculosis strains, but it did not differentiate M. bovis from M, bovis BCG, All M. bovis BCG strains exhibited identical spoligotype patterns. The discriminatory power of spoligotyping of low-copy-number M. tuberculosis strains was higher than that of IS6110 fingerprinting, Based on RFLP typing solely, 83% of the low-copy-number M. tuberculosis strains were found to form part of a cluster, and 75% were found to form a cluster on the basis of spoligotyping, When the two techniques were combined, the amount of clustering decreased to 55%, The combination of these two techniques might be valuable in studying the epidemiology of M, tuberculosis strains harboring few copies of the IS6110 element.