Ubiquitous expression of marker transgenes in mice and rats
Ubiquitous expression of marker transgenes in mice and rats
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DOI：
10.1006/dbio.1999.9417
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发表时间：
1999-10-01
影响因子：
2.7
通讯作者：
Sandgren, EP
中科院分区：
文献类型：
--
作者：
Kisseberth, WC;Brettingen, NT;Sandgren, EP
明确标记细胞基因型的能力对于必须在体内将遗传上不同的细胞群体彼此区分开的研究是必不可少的。应对这一挑战的一种方法是创建表达转基因标记的转基因小鼠，该标记很容易检测，没有背景染色。已经描述了携带具有启动子元件和编码序列的不同组合的构建体的多个转基因小鼠品系，每一个都具有其自身的优点和局限性。在这份报告中，我们描述了使用800 bp的启动子片段分离的β地理整合位点在ROSA 26小鼠靶向表达的两个标记基因。我们证明，ROSA 26启动子指导无处不在的人胎盘碱性磷酸酶和增强型绿色荧光蛋白在小鼠和大鼠胚胎和出生后发育的表达。我们进一步证明了这些转基因在移植研究中标记供体细胞的一般效用。(C)北京：科学出版社.
The ability to unambiguously mark a cell's genotype is essential for studies in which genetically distinct cell populations must be distinguished from one another in vivo. One approach to this challenge has been the creation of transgenic mice expressing a transgene marker that is easily detectable, with no background staining. Multiple transgenic mouse strains bearing constructs with different combinations of promoter elements and coding sequences have been described, each with its own advantages and limitations. In this report we describe the use of an 800-bp promoter fragment isolated from the beta geo integration site in ROSA26 mice to target expression of two marker genes. We demonstrate that the ROSA26 promoter directs ubiquitous expression of human placental alkaline phosphatase and enhanced green fluorescent protein during embryonic and postnatal development in mouse and rat. We further demonstrate the general utility of these transgenes for marking donor cells in transplantation studies. (C) 1999 Academic Press.