The distribution of plasmids that carry virulence and resistance genes in Staphylococcus aureus is lineage associated.
The distribution of plasmids that carry virulence and resistance genes in Staphylococcus aureus is lineage associated.
复制标题
DOI：
10.1186/1471-2180-12-104
复制
发表时间：
2012-06-12
期刊：
影响因子：
4.2
通讯作者：
Lindsay JA
中科院分区：
文献类型：
--
作者：
McCarthy AJ;Lindsay JA
金黄色葡萄球菌是人类和动物的主要致病菌。质粒携带有抗性基因和毒力基因，可通过沙门氏菌传播。金黄色葡萄球菌群体通过水平基因转移（HGT）机制。S.金黄色葡萄球菌质粒和多菌株基因芯片的数据进行了分析，以研究（i）耐药基因和毒力基因在金黄色葡萄球菌中的分布;金黄色葡萄球菌质粒，和（ii）质粒之间的分布。金黄色血统。共21个质粒rep基因家族，其中13个是新的本研究，其特征在于使用以前提出的分类系统。将243个测序质粒分配到39个质粒组中，每个质粒组具有独特的rep基因组合。我们发现一些抗性基因（包括ermC和cat）和毒力基因（包括entA，entG，entJ，entP）与特定的质粒群相关，这表明存在遗传压力，阻止这些基因重组成新的质粒群。全基因组芯片分析表明，质粒rep、抗性基因和毒力基因与S.金黄色葡萄球菌谱系，表明菌株之间存在质粒HGT的限制性修饰（RM）屏障。偶联转移复合体基因很少。这项研究认为，遗传压力抑制了抗性和毒力基因在沙门氏菌中的传播。aureus质粒，而在S.金黄色葡萄球菌种群，延迟完全毒力和耐药菌株的出现。
Staphylococcus aureus is major human and animal pathogen. Plasmids often carry resistance genes and virulence genes that can disseminate through S. aureus populations by horizontal gene transfer (HGT) mechanisms. Sequences of S. aureus plasmids in the public domain and data from multi-strain microarrays were analysed to investigate (i) the distribution of resistance genes and virulence genes on S. aureus plasmids, and (ii) the distribution of plasmids between S. aureus lineages. A total of 21 plasmid rep gene families, of which 13 were novel to this study, were characterised using a previously proposed classification system. 243 sequenced plasmids were assigned to 39 plasmid groups that each possessed a unique combination of rep genes. We show some resistance genes (including ermC and cat) and virulence genes (including entA, entG, entJ, entP) were associated with specific plasmid groups suggesting there are genetic pressures preventing recombination of these genes into novel plasmid groups. Whole genome microarray analysis revealed that plasmid rep, resistance and virulence genes were associated with S. aureus lineages, suggesting restriction-modification (RM) barriers to HGT of plasmids between strains exist. Conjugation transfer (tra) complex genes were rare. This study argues that genetic pressures are restraining the spread of resistance and virulence genes amongst S. aureus plasmids, and amongst S. aureus populations, delaying the emergence of fully virulent and resistant strains.